Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials
| العنوان: | Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials |
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| المؤلفون: | Hazuki Maezono, Reiko Yamamoto, Naomichi Yoneda, Yuichiro Noiri, Shigeyuki Ebisu, Takayoshi Nakano, Mikako Hayashi, Takuya Ishimoto, Katsutaka Kuremoto |
| المصدر: | Applied and Environmental Microbiology. 80:3804-3810 |
| بيانات النشر: | American Society for Microbiology, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Molar, Dentistry, Mesial root, Bacterial Physiological Phenomena, Applied Microbiology and Biotechnology, stomatognathic system, Environmental Microbiology, Animals, Medicine, Periapical Diseases, Rats, Wistar, Tooth Root, Dental Pulp Cavity, Periapical periodontitis, Bacteria, Ecology, business.industry, Biofilm, Bacterial Infections, biochemical phenomena, metabolism, and nutrition, medicine.disease, Bacterial Load, Periapical lesion, Disease Models, Animal, Biofilms, Pulp (tooth), Tomography, X-Ray Computed, business, Food Science, Biotechnology |
| الوصف: | Although extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extraradicular biofilm-forming bacteria while investigating the effect of extraradicular biofilms on periapical lesions. Periapical lesions were induced by exposing the pulpal tissue of the mandibular first molars of male Wistar rats to their oral environment. Four weeks later, gutta-percha points were excessively inserted into the mesial root canals of the right first molars (experimental sites) but not the left first molars (control sites). After 6 and 8 weeks of pulp exposure, the presence of extraradicular biofilms was confirmed histomorphologically, and biofilm-forming bacteria were identified by using classical culture methods. The biofilms were observed in the extraradicular area of the experimental sites. Similar species were detected both inside and outside the root canals. The bacterial count, quantified by real-time PCR assays, in the extraradicular area gradually increased in the experimental sites until 20 weeks after pulp exposure. After 8 weeks of pulp exposure, the periapical lesion volume that was measured by micro-computed tomography was significantly larger in the experimental sites than in the control sites ( P < 0.05 by Welch's t test). These results suggest that we developed an extraradicular biofilm model in rats and that extraradicular biofilms affect developing periapical lesions. |
| تدمد: | 1098-5336 0099-2240 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7afcc68e2f9645b02f1c633f9c8fab81Test https://doi.org/10.1128/aem.00421-14Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7afcc68e2f9645b02f1c633f9c8fab81 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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