Detection of virulence genes ofClostridium difficileby multiplex PCR
| العنوان: | Detection of virulence genes ofClostridium difficileby multiplex PCR |
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| المؤلفون: | Eija Könönen, Martti Vaara, Sointu Mero, Silja Mentula, Tanja Pasanen, Päivi Tissari, Anni‐Riitta Virolainen‐Julkunen, Juha Kirveskari, Eveliina Tarkka, S. M. Kotila, Jenni Antikainen |
| المصدر: | APMIS. 117:607-613 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | DNA, Bacterial, Microbiology (medical), Bacterial Toxins, Virulence, Polymerase Chain Reaction, Sensitivity and Specificity, Pathology and Forensic Medicine, law.invention, Microbiology, Enterotoxins, Bacterial Proteins, law, Genotype, Multiplex polymerase chain reaction, Pulsed-field gel electrophoresis, Humans, Immunology and Allergy, Clostridiaceae, Enterocolitis, Pseudomembranous, Polymerase chain reaction, biology, Clostridioides difficile, General Medicine, Clostridium difficile, biology.organism_classification, Virology, Repressor Proteins, Restriction enzyme |
| الوصف: | Antikainen J, Pasanen T, Mero S, Tarkka E, Kirveskari J, Kotila S, Mentula S, Kononen E, Virolainen-Julkunen A-R, Vaara M, Tissari P. Detection of virulence genes of Clostridium difficile by multiplex PCR. APMIS 2009; 117: 607–13. Clostridium difficile strains belonging to the PCR ribotype 027, pulse-field gel electrophoresis (PFGE) type NAP1, toxinotype III and restriction endonuclease analysis group BI harbouring mutations in the tcdC gene and possessing binary toxin components A and B have been described to cause epidemics with increased morbidity and mortality. In the present study we developed a conventional multiplex PCR designed to detect selected virulence associated markers of the hypervirulent C. difficile PCR ribotype 027. The multiplex PCR assay detected the major toxins A and B, binary toxin components A and B as well as a possible deletion in the tcdC gene: a characteristic pattern of amplification products for the PCR ribotype 027 strains was detected. This rather simple method was specific for the screening of this hypervirulent C. difficile strain. The correlation between the multiplex PCR and PCR ribotyping methods was excellent. The sensitivity and specificity were 100% in our epidemiological situation. In conclusion, this multiplex PCR was found useful in the preliminary screening for the hypervirulent C. difficile PCR ribotype 027. |
| تدمد: | 1600-0463 0903-4641 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8f990e8dfb1078dd73e1e73f2e7618cTest https://doi.org/10.1111/j.1600-0463.2009.02509.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....c8f990e8dfb1078dd73e1e73f2e7618c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16000463 09034641 |
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