Effect of NCAM-transfection on growth and invasion of a human cancer cell line
| العنوان: | Effect of NCAM-transfection on growth and invasion of a human cancer cell line |
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| المؤلفون: | Frank S. Walsh, Nina Pedersen, Mogens Spang-Thomsen, Stana Jirus, Elisabeth Bock, Thomas Frandsen, Claus Moser, Claus Holst-Hansen, Nils Brünner, Klaus Edvardsen, Lars Vindeløv |
| المصدر: | ResearcherID |
| بيانات النشر: | Wiley, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Microbiology (medical), Pathology, medicine.medical_specialty, Transfection, Pathology and Forensic Medicine, Tumor Cells, Cultured, medicine, Humans, Immunology and Allergy, Neoplasm Invasiveness, Fluorescent Antibody Technique, Indirect, Neural Cell Adhesion Molecules, Matrigel, biology, Cell adhesion molecule, Cell growth, Cell Cycle, DNA, Neoplasm, General Medicine, In vitro, Cell biology, Drug Combinations, nervous system, Cell culture, biology.protein, Proteoglycans, Neural cell adhesion molecule, Collagen, Laminin, Antibody |
| الوصف: | A cDNA encoding the human transmembrane 140 kDa isoform of the neural cell adhesion molecule (NCAM) was transfected into the highly invasive MDA-MB-231 human breast cancer cell line. Transfectants with a homogeneous expression of NCAM showed a restricted capacity for penetration of an artificial basement membrane. However, when injected into nude mice, both control and NCAM-expressing cell lines produced equally invasive tumors. Tumors generated from NCAM-transfected cells were heterogeneous, containing NCAM-positive as well as NCAM-negative areas, indicating the existence of host factors capable of modulating NCAM expression in vivo. In nude mice, NCAM-transfected cells developed tumors with longer latency periods and slower growth rates than tumors induced by NCAM-negative control cells, implying that NCAM may be involved not only in adhesive and motile behavior of tumor cells but also in their growth regulation. There was no indication of differences in cell proliferative characteristics between the different NCAM-transfected and the control transfected cells as determined by flow cytometric DNA analysis, suggesting an increased cell loss as the reason for decreased in vivo growth rate of the NCAM-transfected cells. The fact that NCAM expression influences growth regulation attributes a pivotal role to this cell adhesion molecule during ontogenesis and tumor development. |
| تدمد: | 1600-0463 0903-4641 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cd2bc8df249ece83d608a9799d08113Test https://doi.org/10.1111/j.1699-0463.1997.tb05103.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9cd2bc8df249ece83d608a9799d08113 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16000463 09034641 |
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