The 2008–2009 H1N1 influenza virus exhibits reduced susceptibility to antibody inhibition: Implications for the prevalence of oseltamivir resistant variant viruses
| العنوان: | The 2008–2009 H1N1 influenza virus exhibits reduced susceptibility to antibody inhibition: Implications for the prevalence of oseltamivir resistant variant viruses |
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| المؤلفون: | Honglin Chen, Pui Wang, Wai Lan Wu, Wenjun Song, Tianwei Lin, Bobo Wing-Yee Mok, Xi Wen, Kwok-Yung Yuen, Genyan Wang, Yixin Chen, Kwok-Hung Chan, Siu-Ying Lau |
| المصدر: | Antiviral Research. 93:144-153 |
| بيانات النشر: | Elsevier BV, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | viruses, Resistance, Mice, chemistry.chemical_compound, Mice, Inbred BALB/c, Influenza A Virus, H1N1 Subtype, H275Y, Phylogeny, Mice, Inbred BALB C, biology, Immunogenicity, H1N1, Antibody titer, Drug resistance, Viral - genetics, Antibodies, Monoclonal, virus diseases, Middle Aged, Female, Viral proteins - genetics - immunology - metabolism, Antibody, Adult, Oseltamivir, Genotype, Influenza A virus, H1N1 subtype - drug effects - genetics - immunology, Neuraminidase, Antiviral Agents, Article, Virus, Cell Line, Microbiology, Viral Proteins, Young Adult, Orthomyxoviridae Infections, Antigen, Oseltamivir - pharmacology, Orthomyxoviridae infections - immunology, Virology, Drug Resistance, Viral, Animals, Humans, Pharmacology, Hemagglutination assay, Influenza, Antibodies, Monoclonal - immunology, respiratory tract diseases, Neuraminidase - genetics - immunology - metabolism, Amino Acid Substitution, chemistry, Mutation, biology.protein, Antiviral agents - pharmacology |
| الوصف: | A naturally-occurring H275Y oseltamivir resistant variant of influenza A (H1N1) virus emerged in 2007, subsequently becoming prevalent worldwide, via an undetermined mechanism. To understand the antigenic properties of the H275Y variant, oseltamivir resistant and susceptible strains of H1N1 viruses were analyzed by hemagglutination inhibition (HI) and microneutralization assays. HI analysis with H1-positive sera obtained from seasonal flu vaccine immunized and non-immunized individuals, and H1-specific monoclonal antibodies, revealed that resistant strains exhibited a reduced reactivity to these antisera and antibodies in the HI assay, as compared to susceptible strains. Neutralization assay testing demonstrated that oseltamivir resistant H1N1 strains are also less susceptible to antibody inhibition during infection. Mice inoculated with a resistant clinical isolate exhibit 4-fold lower virus-specific antibody titers than mice infected with a susceptible strain under the same conditions. Resistant and sensitive variants of 2009 pandemic H1N1 virus did not exhibit such differences. While HA1 and NA phylogenetic trees show that both oseltamivir resistant and susceptible strains belong to clade 2B, NA D354G and HA A189T substitutions were found exclusively, and universally, in oseltamivir resistant variants. Our results suggest that the reduced susceptibility to antibody inhibition and lesser in vivo immunogenicity of the oseltamivir resistant 2008-2009 H1N1 influenza A virus is conferred by coupled NA and HA mutations, and may contribute to the prevalence of this H1N1 variant. © 2011 Elsevier B.V. link_to_OA_fulltext |
| تدمد: | 0166-3542 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86179addf55142e4c3490470efd56ea3Test https://doi.org/10.1016/j.antiviral.2011.11.006Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....86179addf55142e4c3490470efd56ea3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01663542 |
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