Cyst Nematode Infection Elicits Alteration in the Level of Reactive Nitrogen Species, Protein S-Nitrosylation and Nitration, and Nitrosoglutathione Reductase in Arabidopsis thaliana Roots
| العنوان: | Cyst Nematode Infection Elicits Alteration in the Level of Reactive Nitrogen Species, Protein S-Nitrosylation and Nitration, and Nitrosoglutathione Reductase in Arabidopsis thaliana Roots |
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| المؤلفون: | Ewa Muszyńska, Justyna Fidler, Marta Gietler, Elżbieta Różańska, Beata Prabucka, Iwona Morkunas, Mateusz Labudda |
| المصدر: | Antioxidants Volume 9 Issue 9 Antioxidants, Vol 9, Iss 795, p 795 (2020) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Physiology, Clinical Biochemistry, Glutathione reductase, Reductase, medicine.disease_cause, 01 natural sciences, Biochemistry, peroxynitrite, S-nitrosothiol, Microbiology, redox balance, 03 medical and health sciences, chemistry.chemical_compound, nitric oxide, parasitic diseases, medicine, Plant defense against herbivory, oxidative stress, Arabidopsis thaliana, glutathione peroxidase, Molecular Biology, glutathione reductase, Reactive nitrogen species, non-symbiotic hemoglobin, biology, lcsh:RM1-950, fungi, food and beverages, Cell Biology, biology.organism_classification, medicine.disease, nitrosative stress, lcsh:Therapeutics. Pharmacology, 030104 developmental biology, chemistry, Nematode infection, Heterodera schachtii, Oxidative stress, 010606 plant biology & botany |
| الوصف: | Reactive nitrogen species (RNS) are redox molecules important for plant defense against pathogens. The aim of the study was to determine whether the infection by the beet cyst nematode Heterodera schachtii disrupts RNS balance in Arabidopsis thaliana roots. For this purpose, measurements of nitric oxide (NO), peroxynitrite (ONOO&minus ), protein S-nitrosylation and nitration, and nitrosoglutathione reductase (GSNOR) in A. thaliana roots from 1 day to 15 days post-inoculation (dpi) were performed. The cyst nematode infection caused generation of NO and ONOO&minus in the infected roots. These changes were accompanied by an expansion of S-nitrosylated and nitrated proteins. The enzyme activity of GSNOR was decreased at 3 and 15 dpi and increased at 7 dpi in infected roots, whereas the GSNOR1 transcript level was enhanced over the entire examination period. The protein content of GSNOR was increased in infected roots at 3 dpi and 7 dpi, but at 15 dpi, did not differ between uninfected and infected roots. The protein of GSNOR was detected in plastids, mitochondria, cytoplasm, as well as endoplasmic reticulum and cytoplasmic membranes. We postulate that RNS metabolism plays an important role in plant defense against the beet cyst nematode and helps the fine-tuning of the infected plants to stress sparked by phytoparasitic nematodes. |
| وصف الملف: | application/pdf |
| تدمد: | 2076-3921 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a4bc3810a4539090c308e43399387a4Test https://doi.org/10.3390/antiox9090795Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1a4bc3810a4539090c308e43399387a4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20763921 |
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