Microbial Photoinactivation by Visible Light Results in Limited Loss of Membrane Integrity
| العنوان: | Microbial Photoinactivation by Visible Light Results in Limited Loss of Membrane Integrity |
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| المؤلفون: | Katharina Hoenes, Richard Bauer, Martin Hessling, Barbara Spellerberg |
| المصدر: | Antibiotics Volume 10 Issue 3 Antibiotics, Vol 10, Iss 341, p 341 (2021) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Lysis, photoinactivation, Photoinaktivierung, 030106 microbiology, Pseudomonas fluorescens, Membrane disorders, reactive oxygen species (ROS), Biochemistry, Microbiology, Bacterial cell structure, Viable but nonculturable, Article, 03 medical and health sciences, chemistry.chemical_compound, DDC 570 / Life sciences, ddc:570, Pharmacology (medical), Propidium iodide, General Pharmacology, Toxicology and Pharmaceutics, Staphylococcus carnosus, 405 nm, biology, Plants Photoinhibition, lcsh:RM1-950, biology.organism_classification, 450 nm, lcsh:Therapeutics. Pharmacology, 030104 developmental biology, Infectious Diseases, chemistry, membrane damage, Biophysics, antimicrobial blue light (aBL), membrane permeabilization, Cell envelope, Reactive Oxygen Species, Intracellular |
| الوصف: | Interest in visible light irradiation as a microbial inactivation method has widely increased due to multiple possible applications. Resistance development is considered unlikely, because of the multi-target mechanism, based on the induction of reactive oxygen species by wavelength specific photosensitizers. However, the affected targets are still not completely identified. We investigated membrane integrity with the fluorescence staining kit LIVE/DEAD® BacLight™ on a Gram positive and a Gram negative bacterial species, irradiating Staphylococcus carnosus and Pseudomonas fluorescens with 405 nm and 450 nm. To exclude the generation of viable but nonculturable (VBNC) bacterial cells, we applied an ATP test, measuring the loss of vitality. Pronounced uptake of propidium iodide was only observed in Pseudomonas fluorescens at 405 nm. Transmission electron micrographs revealed no obvious differences between irradiated samples and controls, especially no indication of an increased bacterial cell lysis could be observed. Based on our results and previous literature, we suggest that visible light photoinactivation does not lead to rapid bacterial cell lysis or disruption. However, functional loss of membrane integrity due to depolarization or inactivation of membrane proteins may occur. Decomposition of the bacterial envelope following cell death might be responsible for observations of intracellular component leakage. publishedVersion |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2079-6382 |
| DOI: | 10.3390/antibiotics10030341 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8eff0d7aeb5932a330341cc7089ab8feTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8eff0d7aeb5932a330341cc7089ab8fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20796382 |
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| DOI: | 10.3390/antibiotics10030341 |