دورية أكاديمية
miR-202-5p Inhibits Lipid Metabolism and Steroidogenesis of Goose Hierarchical Granulosa Cells by Targeting ACSL3
| العنوان: | miR-202-5p Inhibits Lipid Metabolism and Steroidogenesis of Goose Hierarchical Granulosa Cells by Targeting ACSL3 |
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| المؤلفون: | Mingxia Ran, Shenqiang Hu, Qingyuan Ouyang, Hengli Xie, Xi Zhang, Yueyue Lin, Xuejian Li, Jiwei Hu, Liang Li, Hua He, Hehe Liu, Jiwen Wang |
| المصدر: | Animals, Vol 13, Iss 3, p 325 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Veterinary medicine LCC:Zoology |
| مصطلحات موضوعية: | miR-202-5p, ACSL3, lipid, steroid hormone, goose granulosa cell, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| الوصف: | miRNAs are critical for steroidogenesis in granulosa cells (GCs) during ovarian follicular development. We have previously shown that miR-202-5p displays a stage-dependent expression pattern in GCs from goose follicles of different sizes, suggesting that this miRNA could be involved in the regulation of the functions of goose GCs; therefore, in this study, the effects of miR-202-5p on lipid metabolism and steroidogenesis in goose hierarchical follicular GCs (hGCs), as well as its mechanisms of action, were evaluated. Oil Red O staining and analyses of intracellular cholesterol and triglyceride contents showed that the overexpression of miR-202-5p significantly inhibited lipid deposition in hGCs; additionally, miR-202-5p significantly inhibited progesterone secretion in hGCs. A bioinformatics analysis and luciferase reporter assay indicated that Acyl-CoA synthetase long-chain family member 3 (ACSL3), which activates long-chain fatty acids for the synthesis of cellular lipids, is a potential target of miR-202-5p. ACSL3 silencing inhibited lipid deposition and estrogen secretion in hGCs. These data suggest that miR-202-5p exerts inhibitory effects on lipid deposition and steroidogenesis in goose hGCs by targeting the ACSL3 gene. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2076-2615 |
| العلاقة: | https://www.mdpi.com/2076-2615/13/3/325Test; https://doaj.org/toc/2076-2615Test |
| DOI: | 10.3390/ani13030325 |
| الوصول الحر: | https://doaj.org/article/931ff368e6fa44a0930935de094d7d12Test |
| رقم الانضمام: | edsdoj.931ff368e6fa44a0930935de094d7d12 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20762615 |
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| DOI: | 10.3390/ani13030325 |