Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification
| العنوان: | Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification |
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| المؤلفون: | Keiko Yamada, Yoshinobu Baba, Mats Nilsson, Jonas Melin, Noritada Kaji, Michio Ohta, Manabu Tokeshi, Laili Mahmoudian, Mohamad Reza Mohamadi |
| المصدر: | Analytical Sciences. 24:327-332 |
| بيانات النشر: | Springer Science and Business Media LLC, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | DNA, Bacterial, Time Factors, medicine.disease_cause, Sensitivity and Specificity, Analytical Chemistry, Bacterial genetics, Electrophoresis, Microchip, chemistry.chemical_compound, medicine, Vibrio cholerae, Gene, biology, Chemistry, Reproducibility of Results, Pathogenic bacteria, biology.organism_classification, Molecular biology, Vibrio, Electrophoresis, genomic DNA, Biochemistry, Microchip Electrophoresis, DNA, Circular, Nucleic Acid Amplification Techniques, DNA |
| الوصف: | We have developed a new method for a fast and precise analysis of circle-to-circle amplification (C2CA) product for specific gene detection by microchip electrophoresis. In this method, we have added a new enzymatic step to the C2CA reaction, which could be carried out isothermally at 37 degrees C. Compared to the original single-stranded DNA, the double-stranded DNA that is produced by this enzymatic reaction is more reliable for analysis by microchip electrophoresis. C2CA product was detected within 55 s with high reproducibility by this method which was successfully applied to the detection of 10-ng genomic DNA of the pathogenic bacteria Vibrio. cholerae within 110 s. Purification was found to be an indispensable step for the analysis of the C2CA product of genomic DNA samples. |
| وصف الملف: | application/pdf |
| تدمد: | 1348-2246 0910-6340 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb79c0b61c2bf0a374c51c77c327382dTest https://doi.org/10.2116/analsci.24.327Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fb79c0b61c2bf0a374c51c77c327382d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13482246 09106340 |
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