Pathogen Identification: Ultrasensitive Nucleic Acid Detection via a Dynamic DNA Nanosystem-Integrated Ratiometric Electrochemical Sensing Strategy
| العنوان: | Pathogen Identification: Ultrasensitive Nucleic Acid Detection via a Dynamic DNA Nanosystem-Integrated Ratiometric Electrochemical Sensing Strategy |
|---|---|
| المؤلفون: | Tao Li, Wei Xing, Fengshan Yu, Ziwei Xue, Xingdong Yang, Guizheng Zou, Ye Zhu |
| المصدر: | Analytical Chemistry. 94:17725-17732 |
| بيانات النشر: | American Chemical Society (ACS), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Limit of Detection, Humans, Nucleic Acid Hybridization, HIV Infections, Electrochemical Techniques, DNA, Biosensing Techniques, Nucleic Acid Amplification Techniques, Analytical Chemistry |
| الوصف: | Sensitively determining trace nucleic acid is of great significance for pathogen identification. Herein, a dynamic DNA nanosystem-integrated ratiometric electrochemical biosensor was proposed to determine human immunodeficiency virus-associated DNA fragment (HIV-DNA) with high sensitivity and selectivity. The dynamic DNA nanosystem was composed of a target recycling unit and a multipedal DNA walker unit. Both of them could be driven by a toehold-mediated strand displacement reaction, enabling an enzyme-free and isothermal amplification strategy for nucleic acid determination. The target recycling unit could selectively recognize HIV-DNA and activate the multipedal DNA walker unit to roll on the electrode surface, which would lead to bidirectional signal variation for ratiometric readout with cascade signal amplification. Benefiting from the synergistic effect of the dynamic DNA nanosystem and the ratiometric output mode, the ultrasensitive detection of HIV-DNA was achieved in a wide linear range of 6 orders of magnitude with a limit of detection of 36.71 aM. The actual usability of the proposed sensor was also verified in complex biological samples with acceptable performance. This dynamic DNA nanosystem-integrated ratiometric sensing strategy might be promising in the development of reliable point-of-care diagnostic devices for highly sensitive and selective pathogen identification. |
| تدمد: | 1520-6882 0003-2700 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::786486f250df01e26e338e7245dcd307Test https://doi.org/10.1021/acs.analchem.2c04736Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....786486f250df01e26e338e7245dcd307 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206882 00032700 |
|---|