Novel Omniphobic Platform for Multicellular Spheroid Generation, Drug Screening, and On-Plate Analysis
| العنوان: | Novel Omniphobic Platform for Multicellular Spheroid Generation, Drug Screening, and On-Plate Analysis |
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| المؤلفون: | Anish Tuteja, Mathew Boban, Geeta Mehta, Taylor Repetto, Alex Kate Halvey, Pooja Mehta |
| المصدر: | Anal Chem |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Chemistry, Extramural, 010401 analytical chemistry, Drug Evaluation, Preclinical, Spheroid, Nanotechnology, Immunofluorescence staining, 010402 general chemistry, 01 natural sciences, Article, Cell aggregation, 0104 chemical sciences, Analytical Chemistry, Contact angle, Neoplasms, Spheroids, Cellular, embryonic structures, Humans, Multicellular spheroid, Drug Screening Assays, Antitumor, Contact area |
| الوصف: | Multicellular spheroids are superior to other culture geometries in reproducing critical physiological conditions of tumors, such as the diffusion of oxygen, nutrients, waste, and drugs, leading to a more precise model of in vivo drug sensitivity and resistance. Previously reported spheroid culture platforms are often difficult to use, expensive, single-use, or mechanically unstable. Here, we report a facile, mechanically stable, high-throughput spheroid culture platform based on hierarchically textured omniphobic surfaces. The developed omniphobic surfaces display very high contact angles with a range of different liquids, including the cell-laden culture media, thereby minimizing the cell surface contact area. Additionally, these surfaces maintain these high contact angles for extended periods of time to ensure cell aggregation. Using this novel platform, we demonstrate the generation and maintenance of robust multicellular spheroids, as well as heterogeneous, multicell-type spheroids. The platform is extremely robust, resistant to mechanical shock, allows for on-plate imaging, and is also the first-ever spheroid generation platform that can be reused repeatedly. Finally, the platform is suitable for on-plate drug screening and enables the first-ever, on-plate immunofluorescence staining and imaging of spheroids. |
| تدمد: | 1520-6882 0003-2700 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a02371df1538e0a4edda68b4eeb2b808Test https://doi.org/10.1021/acs.analchem.1c01326Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a02371df1538e0a4edda68b4eeb2b808 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206882 00032700 |
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