Transmission FT-IR Chemical Imaging on Glass Substrates: Applications in Infrared Spectral Histopathology
| العنوان: | Transmission FT-IR Chemical Imaging on Glass Substrates: Applications in Infrared Spectral Histopathology |
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| المؤلفون: | Peter Gardner, Kaye J. Williams, Paul Bassan, Joe Mellor, Michael P. Lisanti, Jonathan Shapiro |
| المصدر: | Analytical Chemistry. 86:1648-1653 |
| بيانات النشر: | American Chemical Society (ACS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Chemical imaging, Infrared, Chemistry, Analytical chemistry, Infrared spectroscopy, chemistry.chemical_element, Breast Neoplasms, Epithelial Cells, Barium, Molecular Imaging, Analytical Chemistry, symbols.namesake, Fourier transform, Transmission (telecommunications), Spectroscopy, Fourier Transform Infrared, Biomarkers, Tumor, symbols, Breast, Glass, Molecular imaging, Fourier transform infrared spectroscopy, Biomedical engineering |
| الوصف: | Fourier transform-infrared (FT-IR) chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass, making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FT-IR chemical imaging, which has a high-wavenumber transmission window allowing measurement of the C-H, N-H, and O-H stretches occurring at ca. 2500-3800 cm(-1). The "fingerprint" region of the IR spectrum occurring below 1800 cm(-1) is not obtainable; however, we demonstrate that a wealth of information is contained in the high wavenumber range using 71 patients on a breast tissue microarray (TMA) as a model for investigation. Importantly, we demonstrate that the tissue can be classified into four basic tissue cell types and that using just the epithelial cells, reasonable discrimination of normal and malignant tissue can be found. |
| تدمد: | 1520-6882 0003-2700 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::372f2bff022781ebaae6a3db62a0d04aTest https://doi.org/10.1021/ac403412nTest |
| رقم الانضمام: | edsair.doi.dedup.....372f2bff022781ebaae6a3db62a0d04a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206882 00032700 |
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