Applications of Electron−Ion Dissociation Reactions for Analysis of Polycationic Chitooligosaccharides in Fourier Transform Mass Spectrometry
| العنوان: | Applications of Electron−Ion Dissociation Reactions for Analysis of Polycationic Chitooligosaccharides in Fourier Transform Mass Spectrometry |
|---|---|
| المؤلفون: | Roman A. Zubarev, Kim F. Haselmann, V. I. Gorbach, Yu. N. Elkin, Bogdan A. Budnik |
| المصدر: | Analytical Chemistry. 75:5994-6001 |
| بيانات النشر: | American Chemical Society (ACS), 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Crystallography, Collision-induced dissociation, Electron-capture dissociation, Fragmentation (mass spectrometry), Chemistry, Analytical chemistry, Mass spectrometry, Tandem mass spectrometry, Dissociation (chemistry), Fourier transform ion cyclotron resonance, Ion cyclotron resonance, Analytical Chemistry |
| الوصف: | Singly protonated, doubly protonated, and sodiated pentaglucosamide (GlcNAc)(5), oligoglucosamines (GlcN)(m)(), and (GlcN)(3)GlcN(3OH14:0) were analyzed in an FTICR mass spectrometer by electron-ion dissociation reactions and compared to collision activation. The general fragmentation mode was found as the asymmetrical sequence fragments (B(n)() and minor C(n)() ion series) with full sequence coverage. Molecular mass information of each glucosamide or glucosamine residue can be readily obtained from the ion series. Fragmentation by electron capture dissociation revealed additional fragmentation of the N-acetyl moiety compared to sustained off-resonance irradiation collision-activated dissociation (SORI-CAD) and electron-induced dissociation (EID). Sodiated GlcNAc(5) molecular adduct ions were analyzed by EID and compared to CAD. Both techniques provided full sequence coverage. EID was more effective, but CAD resulted in the cross-ring ion products (0,2)A(n)() and (2,4)A(n)() for all relevant glucosamide residues. |
| تدمد: | 1520-6882 0003-2700 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::971be5b409cc9b087838b2d9f7b13fa9Test https://doi.org/10.1021/ac034477fTest |
| رقم الانضمام: | edsair.doi.dedup.....971be5b409cc9b087838b2d9f7b13fa9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206882 00032700 |
|---|