التفاصيل البيبلوغرافية
| العنوان: |
Neutral Red versus MTT assay of cell viability in the presence of copper compounds. |
| المؤلفون: |
Gomez Perez, Mariela1 gomez_perez.mariela@courrier.uqam.ca, Fourcade, Lyvia1 lyvia.fourcade@gmail.com, Mateescu, Mircea Alexandru1 mateescu.m-alexandru@uqam.ca, Paquin, Joanne1 paquin.joanne@uqam.ca |
| المصدر: |
Analytical Biochemistry. Oct2017, Vol. 535, p43-46. 4p. |
| مصطلحات موضوعية: |
*MYOBLAST transfer therapy, *COPPER compounds, *L-Lactate dehydrogenase, *ENZYME activation, *NEURONS |
| مستخلص: |
Copper is essential for numerous physiological functions, and copper compounds may display therapeutic as well as cytotoxic effects. The MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay is a standard test largely used in cytotoxicity studies. This report shows that low micromolar levels of copper compounds such as Cu(II)Urea 2 , Cu(II)Ser 2 and CuCl 2 can interfere with the MTT assay making improper the detection of formazan product of MTT reduction. Comparatively, the Neutral Red assay appears to be sensitive and showing no interference with these compounds. The lactate dehydrogenase alternative assay cannot be used because of inhibitory effect of these copper compounds on the enzyme activity. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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