Formation of S-(carboxymethyl)-cysteine in rat liver mitochondrial proteins: effects of caloric and methionine restriction
| العنوان: | Formation of S-(carboxymethyl)-cysteine in rat liver mitochondrial proteins: effects of caloric and methionine restriction |
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| المؤلفون: | Reinald Pamplona, Mariona Jové, Rosanna Cabré, Gustavo Barja, Victoria Ayala, Pilar Caro, José Ignacio Gómez, Daniel Cacabelos, Manuel Portero-Otin, Alba Naudí |
| المصدر: | Amino Acids. 44:361-371 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Clinical Biochemistry, Lysine, Oxidative phosphorylation, Mitochondrion, Proteomics, medicine.disease_cause, Biochemistry, Mitochondrial Proteins, chemistry.chemical_compound, symbols.namesake, Methionine, medicine, Animals, Rats, Wistar, Caloric Restriction, Molecular Structure, Carbocysteine, Organic Chemistry, Mitochondria, Rats, Oxidative Stress, Maillard reaction, Liver, chemistry, symbols, Oxidative stress, Cysteine |
| الوصف: | Maillard reaction contributes to the chemical modification and cross-linking of proteins. This process plays a significant role in the aging process and determination of animal longevity. Oxidative conditions promote the Maillard reaction. Mitochondria are the primary site of oxidants due to the reactive molecular species production. Mitochondrial proteome cysteine residues are targets of oxidative attack due to their specific chemistry and localization. Their chemical, non-enzymatic modification leads to dysfunctional proteins, which entail cellular senescence and organismal aging. Previous studies have consistently shown that caloric and methionine restrictions, nutritional interventions that increase longevity, decrease the rate of mitochondrial oxidant production and the physiological steady-state levels of markers of oxidative damage to macromolecules. In this scenario, we have detected S-(carboxymethyl)-cysteine (CMC) as a new irreversible chemical modification in mitochondrial proteins. CMC content in mitochondrial proteins significantly correlated with that of the lysine-derived analog N e-(carboxymethyl)-lysine. The concentration of CMC is, however, one order of magnitude lower compared with CML likely due in part to the lower content of cysteine with respect to lysine of the mitochondrial proteome. CMC concentrations decreases in liver mitochondrial proteins of rats subjected to 8.5 and 25 % caloric restriction, as well as in 40 and 80 % methionine restriction. This is associated with a concomitant and significant increase in the protein content of sulfhydryl groups. Data presented here evidence that CMC, a marker of Cys-AGE formation, could be candidate as a biomarker of mitochondrial damage during aging. |
| تدمد: | 1438-2199 0939-4451 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c254f7e44313aada845791db48504a5aTest https://doi.org/10.1007/s00726-012-1339-2Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....c254f7e44313aada845791db48504a5a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14382199 09394451 |
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