Cloning of a Human Platelet-activating Factor Receptor Gene: Evidence for an Intron in the 5′-untranslated Region
| العنوان: | Cloning of a Human Platelet-activating Factor Receptor Gene: Evidence for an Intron in the 5′-untranslated Region |
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| المؤلفون: | John W. Regan, Marilyn Halonen, Peter B. Chase |
| المصدر: | American Journal of Respiratory Cell and Molecular Biology. 8:240-244 |
| بيانات النشر: | American Thoracic Society, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Five prime untranslated region, Guinea Pigs, Molecular Sequence Data, Restriction Mapping, Clinical Biochemistry, Receptors, Cell Surface, Platelet Membrane Glycoproteins, Biology, Polymerase Chain Reaction, Receptors, G-Protein-Coupled, Sequence Homology, Nucleic Acid, Complementary DNA, Leukocytes, Consensus sequence, Animals, Humans, Coding region, Genomic library, Amino Acid Sequence, Cloning, Molecular, Platelet Activating Factor, Codon, Lung, Molecular Biology, Gene, Genetics, Genomic Library, Base Sequence, cDNA library, Intron, DNA, Cell Biology, Molecular biology, Introns, Oligodeoxyribonucleotides, Protein Biosynthesis |
| الوصف: | A clone encoding a gene for a human platelet-activating factor (PAF) receptor has been isolated from a human genomic library. A 6-kb Hind III fragment was subcloned and was found to contain a full coding sequence identical with that previously reported for cDNA clones encoding PAF receptors from leukocyte cDNA libraries. Sequencing of the 6-kb Hind III fragment upstream from the start codon revealed that the 5'-untranslated region deviated from reported cDNA sequences beginning at base -39, suggesting the presence of an intron in this region. Consensus sequences for a splice junction appear appropriately located at the predicted 3' end of the purported intron. Restriction map analysis of the region revealed that the size of the intron in the 5'-untranslated region was at least 16 kb. These data indicate that a gene for a human PAF receptor is present in the genome without introns in the coding sequence and that splicing of mRNA encoding PAF receptors appears to occur in the 5'-untranslated region. |
| تدمد: | 1535-4989 1044-1549 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e9174e0f2e3894b34a3d92b249d9244Test https://doi.org/10.1165/ajrcmb/8.3.240Test |
| رقم الانضمام: | edsair.doi.dedup.....2e9174e0f2e3894b34a3d92b249d9244 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15354989 10441549 |
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