Alterations in the profile of blood neutrophil membrane receptors caused by in vivo adrenocorticotrophic hormone actions
| العنوان: | Alterations in the profile of blood neutrophil membrane receptors caused by in vivo adrenocorticotrophic hormone actions |
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| المؤلفون: | Sandra Helena Poliselli Farsky, Sonia Maria Oliani, Carine Cristiane Drewes, Cristiane Damas Gil, Mauro Perretti, Isabel Daufenback Machado, José Roberto Santin |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | American Physiological Society, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, endocrine system, medicine.medical_specialty, Neutrophils, Surface Properties, Physiology, Endocrinology, Diabetes and Metabolism, INFLAMAÇÃO, Adrenocorticotropic hormone, Biology, CXCR4, Hormone Antagonists, Adrenocorticotropic Hormone, Phagocytosis, Stress, Physiological, In vivo, Cell surface receptor, Physiology (medical), Internal medicine, medicine, Animals, Receptor, Cells, Cultured, Annexin A1, Mice, Knockout, Mice, Inbred BALB C, Macrophages, Cell Membrane, Chemotaxis, Neutrophilia, Up-Regulation, Chemotaxis, Leukocyte, Endocrinology, Receptors, Corticotropin, Leukopoiesis, medicine.symptom, Corticosterone, Stress, Psychological |
| الوصف: | Elevated levels of adrenocorticotrophic hormone (ACTH) mobilize granulocytes from bone marrow into the blood, although these neutrophils are refractory to a full migratory response into inflamed tissues. Here, we investigated the dependence of glucocorticoid receptor activation and glucocorticoid-regulated protein annexin A1 (ANXA1) on ACTH-induced neutrophilia and the phenotype of blood neutrophil after ACTH injection, focusing on adhesion molecule expressions and locomotion properties. ACTH injection (5 μg ip, 4 h) induced neutrophilia in wild-type (WT) mice and did not alter the elevated numbers of neutrophils in RU-38486 (RU)-pretreated or ANXA1−/− mice injected with ACTH. Neutrophils from WT ACTH-treated mice presented higher expression of Ly6G+ANXA1high, CD18high, CD62Lhigh, CD49high, CXCR4high, and formyl-peptide receptor 1 (FPR1low) than those observed in RU-pretreated or ANXA1−/− mice. The membrane phenotype of neutrophils collected from WT ACTH-treated mice was paralleled by elevated fractions of rolling and adherent leukocytes to the cremaster postcapillary venules together with impaired neutrophil migration into inflamed air pouches in vivo and in vitro reduced formyl-methionyl-leucyl-phenylalanine (fMLP) or stromal-derived factor-1 (SDF-1α)-induced chemotaxis. In an 18-h senescence protocol, neutrophils from WT ACTH-treated mice had a higher proportion of ANXAVlow/CXCR4low, and they were less phagocytosed by peritoneal macrophages. We conclude that alterations on HPA axis affect the pattern of membrane receptors in circulating neutrophils, which may lead to different neutrophil phenotypes in the blood. Moreover, ACTH actions render circulating neutrophils to a phenotype with early reactivity, such as in vivo leukocyte-endothelial interactions, but with impaired locomotion and clearance. |
| تدمد: | 1522-1555 0193-1849 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3c0f8452816846e851dc1a7b2a48e0cTest https://doi.org/10.1152/ajpendo.00227.2014Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b3c0f8452816846e851dc1a7b2a48e0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221555 01931849 |
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