No evidence of attenuation of placental insulin-stimulated Akt phosphorylation and amino acid transport in maternal obesity and gestational diabetes mellitus
| العنوان: | No evidence of attenuation of placental insulin-stimulated Akt phosphorylation and amino acid transport in maternal obesity and gestational diabetes mellitus |
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| المؤلفون: | Theresa L. Powell, Thomas Jansson, Marisol Castillo-Castrejon |
| المصدر: | Am J Physiol Endocrinol Metab |
| بيانات النشر: | American Physiological Society, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, medicine.medical_specialty, endocrine system diseases, Physiology, Placenta, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Primary Cell Culture, 030209 endocrinology & metabolism, Fetal Macrosomia, Obesity, Maternal, 03 medical and health sciences, 0302 clinical medicine, Syncytiotrophoblast, Pregnancy, Physiology (medical), Internal medicine, Humans, Hypoglycemic Agents, Insulin, Medicine, Amino Acids, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, chemistry.chemical_classification, 030219 obstetrics & reproductive medicine, business.industry, nutritional and metabolic diseases, medicine.disease, Obesity, female genital diseases and pregnancy complications, Trophoblasts, Amino acid, Gestational diabetes, Diabetes, Gestational, Endocrinology, medicine.anatomical_structure, chemistry, Female, Akt phosphorylation, Chorionic Villi, Insulin Resistance, business, Proto-Oncogene Proteins c-akt, Research Article |
| الوصف: | Pregnancies complicated by obesity and/or gestational diabetes (GDM) are associated with peripheral insulin resistance; however, the insulin responsiveness of the placenta in these pregnancy complications remains largely unknown. We tested the hypothesis that primary human trophoblast cells and placental villous explants will be insulin responsive, characterized by amino acid transport, Akt and Erk activity with maternal obesity, and/or GDM. We evaluated term placentas from women with normal body mass index (BMI) (normal; n = 15), obesity (OB; n = 11), normal BMI with GDM (N-GDM; n = 11), and obesity with GDM (OB-GDM; n = 11). In a subgroup, primary human trophoblast cells (PHT) were isolated, and in an independent subgroup placental villous explants were exposed to varying concentrations of insulin. Amino acid transport capacity and insulin signaling activity were determined. Insulin significantly increased amino acid transport activity to a similar degree in PHT cells isolated from normal (+21%), N-GDM (+38%), OB (+37%), and OB-GDM (+35%) pregnancies. Insulin increased Akt and Erk phosphorylation in PHT cells (3-fold) and in villous explants (2-fold) in all groups to a similar degree. In contrast to the peripheral maternal insulin resistance commonly associated with obesity and/or GDM, we found that the placenta is insulin sensitive in these pregnancy complications. We suggest that elevated maternal insulin levels in pregnancies complicated by obesity and/or GDM promote critical placental functions, including amino acid transport. Insulin-stimulated placental nutrient delivery may contribute to the increased risk of fetal overgrowth and adiposity in these pregnancies. Moreover, our findings may inform efforts to optimize insulin regimens for women with GDM. |
| تدمد: | 1522-1555 0193-1849 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c049437242b81fdbc7e9a6309fe8211cTest https://doi.org/10.1152/ajpendo.00196.2019Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c049437242b81fdbc7e9a6309fe8211c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221555 01931849 |
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