Determination of the membrane topology of lemur tyrosine kinase 2 (LMTK2) by fluorescence protease protection
| العنوان: | Determination of the membrane topology of lemur tyrosine kinase 2 (LMTK2) by fluorescence protease protection |
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| المؤلفون: | Alexander Nixon, Carl White, Neil A. Bradbury, Ying Jia |
| المصدر: | American Journal of Physiology-Cell Physiology. 304:C164-C169 |
| بيانات النشر: | American Physiological Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cytoplasm, Protease, Physiology, medicine.medical_treatment, Green Fluorescent Proteins, Membrane Proteins, Articles, Cell Biology, Protein Serine-Threonine Kinases, Biology, Kidney, LMTK2, Cell biology, Green fluorescent protein, HEK293 Cells, Biochemistry, Membrane protein, Catalytic Domain, Membrane topology, medicine, Humans, Tyrosine, Integral membrane protein |
| الوصف: | Lemur tyrosine kinase 2 (LMTK2) is a novel membrane-anchored kinase reported to be involved in several normal and pathophysiological conditions, including endosomal membrane recycling, prostate cancer, and neurodegeneration. In this study, we have investigated the topology and orientation of LMTK2 within cellular membranes utilizing fluorescence protease protection. Appending the green fluorescent protein to either the amino or carboxyl terminus of LMTK2, we were able to determine which side of intracellular membrane these regions were located. Our results indicate that LMTK2 is an integral membrane protein in which both the amino and carboxyl termini are exposed to the cytoplasm. Moreover, this topology places the kinase active site within the cytoplasm. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31f6556326cf48f14624a7543f3f913eTest https://doi.org/10.1152/ajpcell.00288.2012Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....31f6556326cf48f14624a7543f3f913e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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