Volume-regulated chloride conductance in the LNCaP human prostate cancer cell line
| العنوان: | Volume-regulated chloride conductance in the LNCaP human prostate cancer cell line |
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| المؤلفون: | Yaroslav M. Shuba, P. G. Kostyuk, F. Van Coppenolle, Natalia Prevarskaya, Brigitte Mauroy, Loic Lemonnier, Roman Skryma |
| المصدر: | ResearcherID |
| بيانات النشر: | American Physiological Society, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Anions, Male, medicine.medical_specialty, Cell Membrane Permeability, Patch-Clamp Techniques, Physiology, 8-Bromo Cyclic Adenosine Monophosphate, Protein tyrosine phosphatase, 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, Membrane Potentials, Substrate Specificity, chemistry.chemical_compound, Adenosine Triphosphate, Chloride Channels, Internal medicine, LNCaP, Tumor Cells, Cultured, medicine, Humans, Channel blocker, Patch clamp, Sodium orthovanadate, Ion transporter, Ion Transport, Carcinoma, Prostatic Neoplasms, Tetraethylammonium, Tyrosine phosphorylation, Cell Biology, Protein-Tyrosine Kinases, Calcium Channel Blockers, Molecular biology, Electric Stimulation, Endocrinology, Hypotonic Solutions, Verapamil, chemistry, DIDS, Nitrobenzoates, Potassium, Calcium |
| الوصف: | Patch-clamp recordings were used to study ion currents induced by cell swelling caused by hypotonicity in human prostate cancer epithelial cells, LNCaP. The reversal potential of the swelling-evoked current suggested that Cl−was the primary charge carrier (termed ICl,swell). The selectivity sequence of the underlying volume-regulated anion channels (VRACs) for different anions was Br−≈I−> Cl−> F−> methanesulfonate ≫ glutamate, with relative permeability numbers of 1.26, 1.20, 1.0, 0.77, 0.49, and 0.036, respectively. The current-voltage patterns of the whole cell currents as well as single-channel currents showed moderate outward rectification. Unitary VRAC conductance was determined at 9.6 ± 1.8 pS. Conventional Cl−channel blockers 5-nitro-2-(3-phenylpropylamino)benzoic acid (100 μM) and DIDS (100 μM) inhibited whole cell ICl,swellin a voltage-dependent manner, with the block decreasing from 39.6 ± 9.7% and 71.0 ± 11.0% at +50 mV to 26.2 ± 7.2% and 14.5 ± 6.6% at −100 mV, respectively. Verapamil (50 μM), a standard Ca2+antagonist and P-glycoprotein function inhibitor, depressed the current by a maximum of 15%. Protein tyrosine kinase inhibitors downregulated ICl,swell(genistein with an IC50of 2.6 μM and lavendustin A by 60 ± 14% at 1 μM). The protein tyrosine phosphatase inhibitor sodium orthovanadate (500 μM) stimulated ICl,swellby 54 ± 11%. We conclude that VRACs in human prostate cancer epithelial cells are modulated via protein tyrosine phosphorylation. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dddb4a0cb734af2de7f587e7ec06fa5aTest https://doi.org/10.1152/ajpcell.2000.279.4.c1144Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dddb4a0cb734af2de7f587e7ec06fa5a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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