دورية أكاديمية
Use of GC-IMS for detection of volatile organic compounds to identify mixed bacterial culture medium
| العنوان: | Use of GC-IMS for detection of volatile organic compounds to identify mixed bacterial culture medium |
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| المؤلفون: | Yanyi Lu, Lin Zeng, Min Li, Bowen Yan, Dandan Gao, Bangfu Zhou, Weiping Lu, Qinghua He |
| المصدر: | AMB Express, Vol 12, Iss 1, Pp 1-11 (2022) |
| بيانات النشر: | SpringerOpen, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | GC-IMS, Headspace gas, Identification of bacteria, mVOCs, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract In order to explore the possibility to identify common wound infection bacteria in mixed culture with gas chromatograph-ion migration spectroscopy (GC-IMS), the headspace gas of single and mixed cultures of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were detected and analyzed by GC-IMS system. The bacteria were cultured in thioglycolate medium tubes then transferred to the sampling bottles (indirect method), or directly cultured in the sampling bottles (direct method) to allow accumulation of volatile compounds and facilitate automation. The specific microorganism volatile organic compounds (mVOCs) of the three bacteria were obtained. Some of them have been known to certain substance, for example, ethanol, isoamyl acetate, Phenylacetaldehyde, 2-heptanone etc., while others have not. Principal component analysis (PCA) showed that a higher separability can be achieved with direct method than indirect method. This work indicated that it is possible to identify compound bacteria by detecting specific mVOCs with GC-IMS, and the specific mVOCs should be medium-dependent. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| العلاقة: | https://doaj.org/toc/2191-0855Test |
| DOI: | 10.1186/s13568-022-01367-0 |
| الوصول الحر: | https://doaj.org/article/27331d59679644f4973861753ac29c48Test |
| رقم الانضمام: | edsdoj.27331d59679644f4973861753ac29c48 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21910855 |
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| DOI: | 10.1186/s13568-022-01367-0 |