Microbial biomass in compost during colonization of Agaricus bisporus
| العنوان: | Microbial biomass in compost during colonization of Agaricus bisporus |
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| المؤلفون: | Vos, Aurin, Heijboer, Amber, Boschker, Henricus T. S., Bonnet, Barbara, Lugones, Luis, Wosten, Han, Sub Molecular Microbiology, Molecular Microbiology |
| المصدر: | AMB Express, 7, 1. Springer Verlag AMB Express 7 (2017) AMB Express, 7 AMB Express |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, compost, Gram-positive bacteria, gas chromatography, 030106 microbiology, Biophysics, Microbial biomass, Biomass, Gram negative bacterium, Fungus, engineering.material, chitin, Applied Microbiology and Biotechnology, Wiskundige en Statistische Methoden - Biometris, complex mixtures, laccase, enzyme degradation, Mushroom, 03 medical and health sciences, lignocellulose, Gram positive bacterium, Botany, Colonization, controlled study, Food science, Mathematical and Statistical Methods - Biometris, 2. Zero hunger, nonhuman, biology, Compost, flame ionization detection, fungi, Fungi, Agaricus bisporus, fungal colonization, biology.organism_classification, PE&RC, enzyme activity, 030104 developmental biology, engineering, Basidiomycete, Original Article, lipids (amino acids, peptides, and proteins), fatty acid, fungal biomass, Bacteria |
| الوصف: | Agaricus bisporus mushrooms are commercially produced on a microbe rich compost. Here, fungal and bacterial biomass was quantified in compost with and without colonization by A. bisporus. Chitin content, indicative of total fungal biomass, increased during a 26-day period from 576 to 779 nmol N-acetylglucosamine g−1 compost in the absence of A. bisporus (negative control). A similar increase was found in the presence of this mushroom forming fungus. The fungal phospholipid-derived fatty acid (PLFA) marker C18:2ω6, indicative of the living fraction of the fungal biomass, decreased from 575 to 280 nmol g−1 compost in the negative control. In contrast, it increased to 1200 nmol g−1 compost in the presence of A. bisporus. Laccase activity was absent throughout culturing in the negative control, while it correlated with the fungal PLFA marker in the presence of A. bisporus. PLFA was also used to quantify living bacterial biomass. In the negative control, the bacterial markers remained constant at 3000–3200 nmol PLFA g−1 compost. In contrast, they decreased to 850 nmol g−1 compost during vegetative growth of A. bisporus, implying that bacterial biomass decreased from 17.7 to 4.7 mg g−1 compost. The relative amount of the Gram positive associated PLFA markers a15:0 and a17:0 and the Gram negative PLFA associated markers cy17:0 and cy19:0 increased and decreased, respectively, suggesting that Gram negative bacteria are more suppressed by A. bisporus. Together, these data indicate that fungal biomass can make up 6.8% of the compost after A. bisporus colonization, 57% of which being dead. Moreover, results show that A. bisporus impacts biomass and composition of bacteria in compost. Electronic supplementary material The online version of this article (doi:10.1186/s13568-016-0304-y) contains supplementary material, which is available to authorized users. |
| وصف الملف: | image/pdf; application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 1094-4087 2191-0855 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f3226805f9152369a9b482ee59f1e3eTest https://doi.org/10.1186/s13568-016-0304-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1f3226805f9152369a9b482ee59f1e3e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10944087 21910855 |
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