Blood eosinophil count and airway epithelial transcriptome relationships in COPD versus asthma
| العنوان: | Blood eosinophil count and airway epithelial transcriptome relationships in COPD versus asthma |
|---|---|
| المؤلفون: | Matthew Richardson, Bertrand De-Meulder, John H. Riley, Anne Boland, Gian Andri Thun, Charles Auffray, Stewart Bates, Anna Esteve-Codina, María Soler Artigas, Wim Timens, Timothy S. C. Hinks, David G. Parr, Maarten van den Berge, Ivo Gut, Per Venge, Dave Singh, Christopher E. Brightling, Martin D. Tobin, Ratko Djukanovic, Leena George, Timm Greulich, Kian Fan Chung, Jens M. Hohlfeld, Antje Prasse, Stelios Pavlidis, Sally E. Wenzel, Ian M. Adcock, Scott Wagers, Piera Boschetto, Pieter S. Hiemstra, Loems Ziegler-Heitbrock, Lindsay M. Edwards, Adam Nowinski, Sven Erik-Dahlen, Simon Heath, Peter J. Sterk, Salman Siddiqui, Adam Taylor, Imre Barta |
| المساهمون: | National Institute for Health Research, Groningen Research Institute for Asthma and COPD (GRIAC), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Pulmonology, Publica |
| المصدر: | Allergy 75, 370-380 (2020) Allergy Allergy, 75(2), 370-380. Wiley-Blackwell Allergy, 75(2), 370-380. WILEY Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Allergy: European Journal of Allergy and Clinical Immunology, 75(2), 370-380. Wiley-Blackwell |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, U-BIOPRED and the EvA study teams, 0301 basic medicine, Allergy, Respiratory Medicine and Allergy, Transcriptome, Leukocyte Count, Pulmonary Disease, Chronic Obstructive, 0302 clinical medicine, T2-immunity, Immunology and Allergy, Prospective Studies, RNA-Seq, Lungmedicin och allergi, COPD, POST-HOC ANALYSIS, Middle Aged, Asthma, Chronic Obstructive Pulmonary Disease, Eosinophil, Gene Expression, respiratory system, 3. Good health, medicine.anatomical_structure, 1107 Immunology, Original Article, Female, medicine.symptom, Life Sciences & Biomedicine, medicine.drug, PHASE, Immunology, Eosinòfils, Respiratory Mucosa, Asthma and Lower Airway Disease, OBSTRUCTIVE PULMONARY-DISEASE, NO, chronic obstructive pulmonary disease, 03 medical and health sciences, T2‐immunity, Th2 Cells, SPUTUM, medicine, Humans, eosinophil, Asma, Aged, Science & Technology, Lung, MEPOLIZUMAB, business.industry, Pulmons -- Malalties, Immunoglobulin E, asthma, medicine.disease, Expressió gènica, SECONDARY ANALYSIS, respiratory tract diseases, Eosinophils, EXACERBATIONS, Immunitat, 030104 developmental biology, 030228 respiratory system, asthma, chronic obstructive pulmonary disease, eosinophil, gene expression, T2-immunity, gene expression, Sputum, ORIGINAL ARTICLES, business, Mepolizumab, Biomarkers, LUNG |
| الوصف: | Background Whether the clinical or pathophysiologic significance of the “treatable trait” high blood eosinophil count in COPD is the same as for asthma remains controversial. We sought to determine the relationship between the blood eosinophil count, clinical characteristics and gene expression from bronchial brushings in COPD and asthma. Methods Subjects were recruited into a COPD (emphysema versus airway disease [EvA]) or asthma cohort (Unbiased BIOmarkers in PREDiction of respiratory disease outcomes, U‐BIOPRED). We determined gene expression using RNAseq in EvA (n = 283) and Affymetrix microarrays in U‐BIOPRED (n = 85). We ran linear regression analysis of the bronchial brushings transcriptional signal versus blood eosinophil counts as well as differential expression using a blood eosinophil > 200 cells/μL as a cut‐off. The false discovery rate was controlled at 1% (with continuous values) and 5% (with dichotomized values). Results There were no differences in age, gender, lung function, exercise capacity and quantitative computed tomography between eosinophilic versus noneosinophilic COPD cases. Total serum IgE was increased in eosinophilic asthma and COPD. In EvA, there were 12 genes with a statistically significant positive association with the linear blood eosinophil count, whereas in U‐BIOPRED, 1197 genes showed significant associations (266 positive and 931 negative). The transcriptome showed little overlap between genes and pathways associated with blood eosinophil counts in asthma versus COPD. Only CST1 was common to eosinophilic asthma and COPD and was replicated in independent cohorts. Conclusion Despite shared “treatable traits” between asthma and COPD, the molecular mechanisms underlying these clinical entities are predominately different. In a chronic obstructive pulmonary disease (COPD) cohort (EvA, n = 283), 12 genes, whereas in asthma cohort (UBIOPRED, n = 85), 1197 genes in bronchial epithelial brushes were correlated with a blood eosinophil count. The gene CST1 was common to eosinophilic asthma and COPD and was replicated in independent cohorts. Despite shared “treatable traits” between asthma and COPD, the molecular mechanisms underlying these clinical entities are predominately different. |
| وصف الملف: | application/pdf |
| تدمد: | 1398-9995 0105-4538 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f049340a29a36b002dfb535b3157b4bTest https://doi.org/10.1111/all.14016Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9f049340a29a36b002dfb535b3157b4b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13989995 01054538 |
|---|