Evaluation of HIV Type 1 Western Blot-Indeterminate Blood Donors for the Presence of Human or Bovine Retroviruses
| العنوان: | Evaluation of HIV Type 1 Western Blot-Indeterminate Blood Donors for the Presence of Human or Bovine Retroviruses |
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| المؤلفون: | MICHAEL P. SHERMAN, NANCY L. DOCK, GARTH D. EHRLICH, JOHN J. SNINSKY, CHERYL BROTHERS, JANICE GILLSDORF, VIRGINIA BRYZ-GORNIA, BERNARD J. POIESZ |
| المصدر: | AIDS Research and Human Retroviruses. 11:409-414 |
| بيانات النشر: | Mary Ann Liebert Inc, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Immunodeficiency Virus, Bovine, Blotting, Western, Molecular Sequence Data, Immunology, New York, Gene Products, gag, Blood Donors, Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction, Virus, Acquired immunodeficiency syndrome (AIDS), Western blot, Risk Factors, HIV Seronegativity, Virology, Immunopathology, HIV Seropositivity, medicine, Animals, Humans, False Positive Reactions, Sida, False Negative Reactions, DNA Primers, Acquired Immunodeficiency Syndrome, Human T-lymphotropic virus 1, Base Sequence, biology, medicine.diagnostic_test, Human T-lymphotropic virus 2, Reproducibility of Results, Bovine immunodeficiency virus, biology.organism_classification, medicine.disease, Genes, gag, Milk, Infectious Diseases, HIV-2, HIV-1, biology.protein, Cattle, Female, Viral disease, Antibody, Retroviridae Infections |
| الوصف: | From 1985 through 1990, 1100 of 500,000 human blood donations in Syracuse, New York were repeatedly reactive by ELISA for antibodies to the human immunodeficiency virus type 1 (HIV-1). Nine hundred of the ELISA-reactive samples were confirmed as negative by Western blot (WB), 40 were confirmed as positive, and the remaining 160 sera were indeterminate, reacting mainly with HIV-1 gag gene products. Twenty donors with the most reactive indeterminate WB were selected for follow-up studies. Four of these 20 donors admitted to retroviral risk factors and, interestingly, 12 (60%) had exposure to dairy cattle and drank unpasteurized milk. These 20 donors were analyzed over a 3-year period for the presence of the pathogenic human retroviruses HIV-1, HIV-2, human T cell lymphoma/leukemia virus types I and II (HTLV-I and HTLV-II), as well as bovine immunodeficiency virus (BIV) and leukemia virus (BLV). Retroviral analyses included serology, plasma antigen capture, virus culture, and the polymerase chain reaction. Only one donor seroconverted and was clearly infected with HIV-1. None of the other 19 donor serological reactivities to HIV-1 changed, nor were they positive for any of the above-mentioned retroviruses. Although we cannot ascertain whether these latter 19 HIV-1 WB-indeterminate donors were exposed to human or bovine retroviral proteins, it is unlikely that their HIV-1 seroreactivity was caused by infection with HIV-1, HIV-2, HTLV-I, HTLV-II, BLV, or BIV. |
| تدمد: | 1931-8405 0889-2229 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54545a78f0eb6e743fd2acd686b5c834Test https://doi.org/10.1089/aid.1995.11.409Test |
| رقم الانضمام: | edsair.doi.dedup.....54545a78f0eb6e743fd2acd686b5c834 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19318405 08892229 |
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