التفاصيل البيبلوغرافية
| العنوان: |
A Comparison of the Effects of Pentoxifylline on Quality of Fresh and Frozen-thawed Bull Spermatozoa. |
| المؤلفون: |
Jian ZHANG, Huamin GUO, Jie SU, Lixia ZHAO, Yunxia LI, Wei SUN, Hongmei HAN, Shuxiang HU, Gaoping ZHAO, Yao LI, Yanfeng DAI, Xihe LI |
| المصدر: |
Agricultural Biotechnology (2164-4993); Jun2014, Vol. 3 Issue 3, p20-29, 6p |
| مصطلحات موضوعية: |
SPERM motility, PENTOXIFYLLINE, BULLS, SPERMATOZOA, ACROSOMES |
| مستخلص: |
[Object] This study aimed to examine the effects of 3,7-dimethyl-1-(5-oxo-hexyl)-xanthine (pentoxifylline, PF) on the motility, mitochondrial activity, acrosome integrity and fertilization rate of spermatozoa in both fresh and frozen-thawed bull semen. [Method] Fresh and frozen-thawed bull spermatozoa were exposed to 5 mmol/L PF with untreated samples as controls. [Result] Fresh spermatozoa showed reduced (P < 0. 05) motility after 2 h incubation with PF whereas, surprisingly, frozen-thawed samples exhibited increased sperm motility (P < 0.05) after 2 h incubation with PF and they also showed enhanced longevity compared to controls. Mitochondrial activity in both fresh and frozen-thawed bull spermatozoa increased (P < 0.05) during 4 h incubation with PF whereas acrosome integrity remained unchanged in both types of semen. However, treatment with 5 mmol/L PF did not influence the in vitro fertilization efficiency of fresh spermatozoa but improved significantly (P < 0.05) that of frozen-thawed spermatozoa. [Conclusion] These results indicate that PF can improve sperm quality of frozen-thawed bull semen, and may improve pregnancy rates in bovine artificial insemination programmes employing frozen semen. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
