دورية أكاديمية
Thymoquinone Promotes Pancreatic Cancer Cell Death and Reduction of Tumor Size through Combined Inhibition of Histone Deacetylation and Induction of Histone Acetylation
| العنوان: | Thymoquinone Promotes Pancreatic Cancer Cell Death and Reduction of Tumor Size through Combined Inhibition of Histone Deacetylation and Induction of Histone Acetylation |
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| المؤلفون: | Daniel Relles, Galina I. Chipitsyna, Qiaoke Gong, Charles J. Yeo, Hwyda A. Arafat |
| المصدر: | Advances in Preventive Medicine, Vol 2016 (2016) |
| بيانات النشر: | Hindawi Limited, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Public aspects of medicine |
| مصطلحات موضوعية: | Public aspects of medicine, RA1-1270 |
| الوصف: | Pancreatic ductal adenocarcinoma (PDAC) is virtually therapy-resistant. As noninvasive lesions progress to malignancy, the precursor period provides a window for cancer therapies that can interfere with neoplastic progression. Thymoquinone (Tq), a major bioactive component of essential oil from Nigella sativa’s seeds, has demonstrated antineoplastic activities in multiple cancers. In this study, we investigated antineoplastic potential of Tq in human PDAC cell lines, AsPC-1 and MiaPaCa-2. Tq (10–50 μM) inhibited cell viability and proliferation and caused partial G2 cycle arrest in dose-dependent manner in both cell lines. Cells accumulated in subG0/G1 phase, indicating apoptosis. This was associated with upregulation of p53 and downregulation of Bcl-2. Independently of p53, Tq increased p21 mRNA expression 12-fold. Tq also induced H4 acetylation (lysine 12) and downregulated HDACs activity, reducing expression of HDACs 1, 2, and 3 by 40–60%. In vivo, Tq significantly reduced tumor size in 67% of established tumor xenografts (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2090-3480 2090-3499 |
| العلاقة: | https://doaj.org/toc/2090-3480Test; https://doaj.org/toc/2090-3499Test |
| DOI: | 10.1155/2016/1407840 |
| الوصول الحر: | https://doaj.org/article/8439507e89494ed885499882a491ca96Test |
| رقم الانضمام: | edsdoj.8439507e89494ed885499882a491ca96 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20903480 20903499 |
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| DOI: | 10.1155/2016/1407840 |