دورية أكاديمية
Optimized Condition for Enhanced Soluble-Expression of Recombinant Mutant Anabaena Variabilis Phenylalanine Ammonia Lyase
| العنوان: | Optimized Condition for Enhanced Soluble-Expression of Recombinant Mutant Anabaena Variabilis Phenylalanine Ammonia Lyase |
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| المؤلفون: | Hossein Zarei Jaliani, Safar Farajnia, Yaghoub Safdari, Seyyed Abolghasem Mohammadi, Abolfazl Barzegar, Saeed Talebi |
| المصدر: | Advanced Pharmaceutical Bulletin, Vol 4, Iss 3, Pp 261-266 (2014) |
| بيانات النشر: | Tabriz University of Medical Sciences, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | Phenylalanine ammonia lyase, Soluble expression, Optimization, Specific activity, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Purpose: Recently discovered Anabaena variabilis phenylalanine ammonia lyase (AvPAL) proved to be a good candidate for enzyme replacement therapy of phenylketonuria. Outstanding stability properties of a mutant version of this enzyme, produced already in our laboratory, have led us to the idea of culture conditions optimization for soluble expression of this therapeutically valuable enzyme in E. coli. Methods: In the present study, the gene encoding mutant version of AvPAL was cloned into the pET28a expression vector. Different concentrations of IPTG, induction period, growth temperature, shaking speed, as well as different types of culture media were examined with respect to the amount of recombinant protein produced and specific activity of the enzyme. Results: Based upon our findings, maximum amount of active mutant enzyme was attained by addition of 0.5 mM IPTG at 150 rpm to the TB culture media. The yield of active enzyme at cluture tempreature of 25 °C and induction period of 18 hour was the highest. Conclusion: The results of this study indicated that the yield of mutant AvPAL production in E. coli can be affected mainly by culture temperature and inducer concentration. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2228-5881 2251-7308 |
| العلاقة: | http://journals.tbzmed.ac.ir/APB/Abstract/APB_1354_20131215112132Test; https://doaj.org/toc/2228-5881Test; https://doaj.org/toc/2251-7308Test |
| DOI: | 10.5681/apb.2014.038 |
| الوصول الحر: | https://doaj.org/article/addd6b11eaac4eeea1943af9ec797af8Test |
| رقم الانضمام: | edsdoj.6b11eaac4eeea1943af9ec797af8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22285881 22517308 |
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| DOI: | 10.5681/apb.2014.038 |