Supramolecular Peptide Nanofibrils with Optimized Sequences and Molecular Structures for Efficient Retroviral Transduction
| العنوان: | Supramolecular Peptide Nanofibrils with Optimized Sequences and Molecular Structures for Efficient Retroviral Transduction |
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| المؤلفون: | Stefanie Sieste, Manuel Hayn, Thomas Mack, Desiree Schütz, Kübra Kaygisiz, Tuomas P. J. Knowles, Annika Röcker, Edina Lump, Christopher V. Synatschke, Francesco Simone Ruggeri, Frank Kirchhoff, Jan Münch, Tanja Weil, Christoph Meier |
| المساهمون: | Weil, T [0000-0002-5906-7205], Apollo - University of Cambridge Repository |
| المصدر: | Advanced Functional Materials 31 (2021) 17 Advanced Functional Materials Advanced Functional Materials, 31(17) |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | assembly, DDC 540 / Chemistry & allied sciences, structure–activity relationship, Materials science, Self-assembly (Chemistry), retroviral gene transfer, self‐, Supramolecular chemistry, Sequence (biology), Peptide, Selbstorganisation, Fibril, structure–, Biomaterials, Transduction (genetics), DDC 620 / Engineering & allied operations, Electrochemistry, Structure–activity relationship, ddc:530, Gene, peptide nanofibrils, activity relationship, chemistry.chemical_classification, structure–, DDC 530 / Physics, Structure-activity relationships (Biochemistry), Organic Chemistry, self‐, self-assembly, Condensed Matter Physics, Organische Chemie, Electronic, Optical and Magnetic Materials, chemistry, ddc:540, Biophysics, Protein quaternary structure, ddc:620 |
| الوصف: | Funder: German Research Foundation; Id: http://dx.doi.org/10.13039/501100001659Test Amyloid‐like peptide nanofibrils (PNFs) are abundant in nature providing rich bioactivities and playing both functional and pathological roles. The structural features responsible for their unique bioactivities are, however, still elusive. Supramolecular nanostructures are notoriously challenging to optimize, as sequence changes affect self‐assembly, fibril morphologies, and biorecognition. Herein, the first sequence optimization of PNFs, derived from the peptide enhancing factor‐C (EF‐C, QCKIKQIINMWQ), for enhanced retroviral gene transduction via a multiparameter and a multiscale approach is reported. Retroviral gene transfer is the method of choice for the stable delivery of genetic information into cells offering great perspectives for the treatment of genetic disorders. Single fibril imaging, zeta potential, vibrational spectroscopy, and quantitative retroviral transduction assays provide the structure parameters responsible for PNF assembly, fibrils morphology, secondary and quaternary structure, and PNF‐virus‐cell interactions. Optimized peptide sequences such as the 7‐mer, CKFKFQF, have been obtained quantitatively forming supramolecular nanofibrils with high intermolecular β‐sheet content that efficiently bind virions and attach to cellular membranes revealing efficient retroviral gene transfer. |
| وصف الملف: | application/pdf; text/xml |
| تدمد: | 1616-3028 1616-301X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97183afe9822247598e895e555db1031Test https://doi.org/10.1002/adfm.202009382Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....97183afe9822247598e895e555db1031 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16163028 1616301X |
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