Chronic activation of neutral ceramidase protects β-cells against cytokine-induced apoptosis
| العنوان: | Chronic activation of neutral ceramidase protects β-cells against cytokine-induced apoptosis |
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| المؤلفون: | Cui-ping Liu, Kuanfeng Xu, Xiao-Hong Shan, Qun Zhu, Junfei Jin, Xiaodong Mao, Chao Liu |
| المصدر: | Acta Pharmacologica Sinica. 29:593-599 |
| بيانات النشر: | Springer Science and Business Media LLC, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | medicine.medical_specialty, Time Factors, medicine.medical_treatment, Apoptosis, Biology, Culture Media, Serum-Free, Gene Expression Regulation, Enzymologic, Cell Line, Flow cytometry, Necrosis, chemistry.chemical_compound, Annexin, Insulin-Secreting Cells, Neutral Ceramidase, Internal medicine, medicine, Animals, Pharmacology (medical), RNA, Messenger, Propidium iodide, Annexin A5, Coloring Agents, Fluorescent Dyes, Pharmacology, medicine.diagnostic_test, General Medicine, Transfection, Molecular biology, Recombinant Proteins, Rats, Enzyme Activation, Endocrinology, Cytokine, chemistry, Cell culture, Cytokines, Fluorescein-5-isothiocyanate, Propidium |
| الوصف: | Aim: To investigate the activity and expression of neutral ceramidase (N-CDase) in the insulin-secreting cell line INS-1 and its role in the cellular response to cytokines. Methods: HPLC, Western blotting, and quantitative real-time PCR were performed to detect the activity and expression of N-CDase in INS-1 cells treated with a cytokine mixture (5 ng/mL interleukin-1β, 10 ng/mL TNF-α, and 50 ng/mL interferon-γ). The expression and activity of N-CDase in the INS-1 cells were specifically inhibited using N-CDase-siRNA transfection. Annexin V-fluorescein-isothiocyanate/propidium iodide flow cytometry was used to assess apoptosis in the INS-1 cells. Results: The INS-1 cells exhibited some basal N-CDase activity, and cytokines induced a time-dependent delay in the activation of N-CDase. As a result, the activation of N-CDase was first detectable at 8 h after stimulation. It peaked at 16 h and remained elevated at 24 h. Cytokines also upregulated the mRNA and protein expression of N-CDase in the INS-1 cells. Furthermore, when N-CDase activity was inhibited by RNA interference, cytokine-induced apoptosis in the INS-1 cells was markedly increased. Conclusion: The N-CDase pathway is active in INS-1 cells, and the chronic activation of N-CDase is involved in the pathological response of β-cells to cytokines, potentially providing protection against cytokine toxicity. |
| تدمد: | 1745-7254 1671-4083 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::909350bea44835006f6faba498b8a844Test https://doi.org/10.1111/j.1745-7254.2008.00781.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....909350bea44835006f6faba498b8a844 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17457254 16714083 |
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