التفاصيل البيبلوغرافية
| العنوان: |
Genomic differences between retinoma and retinoblastoma. |
| المؤلفون: |
Sampieri, Katia, Mencarelli, Maria Antonietta, Carmela Epistolato, Maria, Toti, Paolo, Lazzi, Stefano, Bruttini, Mirella, Francesco, Sonia De, Longo, Ilaria, Meloni, Ilaria, Mari, Francesca, Acquaviva, Antonio, Hadjistilianou, Theodora, Renieri, Alessandra, Ariani, Francesca |
| المصدر: |
Acta Oncologica; Dec2008, Vol. 47 Issue 8, p1483-1492, 10p, 3 Color Photographs, 1 Diagram, 1 Chart, 1 Graph |
| مصطلحات موضوعية: |
RETINOBLASTOMA, RETINA, GENOMICS, NEUROBLASTOMA, CANCER invasiveness, TUMORS |
| مستخلص: |
Introduction. Genomic copy number changes are involved in the multi-step process transforming normal retina in retinoblastoma after RB1 mutational events. Previous studies on retinoblastoma samples led to a multi-step model in which after two successive RB1 mutations, further genomic changes accompany malignancy: 1q32.1 gain is followed by 6p22 gain, that in turn is followed by 16q22 loss and 2p24.1 gain. Retinoma is a benign variant of retinoblastoma that was initially considered a tumor regression, but recent evidences suggest that it rather represents a pre-malignant lesion. Genetic studies on retinoma tissue have rarely been performed. Materials and methods. We investigated by Real-Time qPCR, copy number changes of candidate genes located within the 4 hot-spot regions (MDM4 at 1q32.1, MYCN at 2p24.1, E2F3 at 6p22 and CDH11 at 16q22) in retina, retinoma and retinoblastoma tissues from two different patients. Results. Our results demonstrated that some copy number changes thought to belong to early (MDM4 gain) or late stage (MYCN and E2F3 gain) of retinoblastoma are already present in retinoma at the same (for MDM4) or at lower (for MYCN and E2F3) copy number variation respect to retinoblastoma. CDH11 copy number is not altered in the two retinoma samples, but gain is present in one of the two retinoblastomas. Discussion. Our results suggest that MDM4 gain may be involved in the early transition from normal retina to retinoma, while MYCN and E2F3 progressive gain may represent driving factors of tumor progression. These results also confirm the pre-malignant nature of retinoma. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
