Expression pattern of apoptosis-related markers in Huntington's disease
| العنوان: | Expression pattern of apoptosis-related markers in Huntington's disease |
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| المؤلفون: | Ellis Schipper, Roelie T de Boer-van Huizen, Marcel M. Verbeek, Pieter Wesseling, Hans J. ten Donkelaar, Robert M.W. de Waal, Berry Kremer, José C Vis |
| المصدر: | Acta Neuropathologica, 109(3), 321-328. SPRINGER Acta Neuropathologica, 109, 3, pp. 321-8 Acta Neuropathologica, 109, 321-8 |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Male, Poly (ADP-Ribose) Polymerase-1, Apoptosis, Neuroinformatics [DCN 3], Collagen Type XI, Models, Perception and Action [DCN 1], 80 and over, Non-U.S. Gov't, bcl-2-Associated X Protein, Regulation of gene expression, Aged, 80 and over, Neurons, TUNEL assay, biology, Glial fibrillary acidic protein, Caspase 3, Research Support, Non-U.S. Gov't, Neurodegeneration, Middle Aged, Immunohistochemistry, medicine.anatomical_structure, Huntington Disease, Proto-Oncogene Proteins c-bcl-2, Caspases, Neuroglia, Female, Poly(ADP-ribose) Polymerases, Functional Neurogenomics [DCN 2], Chemical and physical biology [NCMLS 7], Adult, Research Support, Models, Biological, Pathology and Forensic Medicine, Cellular and Molecular Neuroscience, Bcl-2-associated X protein, Huntington's disease, Cognitive neurosciences [UMCN 3.2], Translational research [ONCOL 3], Glial Fibrillary Acidic Protein, medicine, In Situ Nick-End Labeling, Journal Article, Humans, Comparative Study, Alzheimer Centre [NCEBP 11], Aged, Immunotherapy, gene therapy and transplantation [UMCN 1.4], medicine.disease, Tissue engineering and pathology [NCMLS 3], Biological, Molecular biology, Genetic defects of metabolism [UMCN 5.1], Gene Expression Regulation, Postmortem Changes, biology.protein, Neurology (clinical), Biomarkers |
| الوصف: | Contains fulltext : 48467.pdf (Publisher’s version ) (Closed access) Inappropriate apoptosis has been implicated in the mechanism of neuronal death in Huntington's disease (HD). In this study, we report the expression of apoptotic markers in HD caudate nucleus (grades 1-4) and compare this with controls without neurological disease. Terminal transferase-mediated biotinylated-UTP nick end-labeling (TUNEL)-positive cells were detected in both control and HD brains. However, typical apoptotic cells were present only in HD, especially in grade 3 and 4 specimens. Expression of the pro-apoptotic protein Bax was increased in HD brains compared to controls, demonstrating a cytoplasmic expression pattern in predominantly shrunken and dark neurons, which were most frequently seen in grades 2 and 3. Control brains displayed weak perinuclear expression of the anti-apoptotic protein Bcl-2, whereas in HD brains Bcl-2 immunoreactivity was markedly enhanced, especially in severely affected grade 4 brains, and was observed in both healthy neurons and dark neurons. Caspase-3, an executioner protease, was only found in four HD brains of different grades and was not expressed in controls. A strong neuronal and glial expression of poly(ADP-ribose) polymerase (PARP)-immunoreactivity was observed in HD brains. These data strongly suggest the involvement of apoptosis in HD. The exact apoptotic pathway occurring in HD neurodegeneration remains yet unclear. However, the presence of late apoptotic events, such as enhanced PARP expression and many TUNEL-positive cells accompanied with weak caspase-3 immunoreactivity in severely affected HD brains, suggests that caspase-mediated neuronal death only plays a minor role in HD. |
| وصف الملف: | application/pdf |
| تدمد: | 0001-6322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69a47660e613400097fa5001b5ae439dTest https://hdl.handle.net/2066/48467Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....69a47660e613400097fa5001b5ae439d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00016322 |
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