Expression of glucose transporters in duodenal mucosa of patients with type 1 diabetes
| العنوان: | Expression of glucose transporters in duodenal mucosa of patients with type 1 diabetes |
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| المؤلفون: | Emanuele Bosi, Riccardo Bonfanti, Giulia Martina Cavestro, Raffaella Alessia Zuppardo, Valeria Sordi, Graziano Barera, Silvia Pellegrini, Amelia Caretto, Andrea Mario Bolla, Elena Butera |
| المساهمون: | Bolla, A. M., Butera, E., Pellegrini, S., Caretto, A., Bonfanti, R., Zuppardo, R. A., Barera, G., Cavestro, G. M., Sordi, V., Bosi, E. |
| المصدر: | Acta Diabetologica. 57:1367-1373 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Biopsy, Endocrinology, Diabetes and Metabolism, Type 2 diabetes, 030204 cardiovascular system & hematology, SGLT2, SGLT1, 0302 clinical medicine, Endocrinology, Gene expression, Intestinal Mucosa, Child, Glucose Transporter Type 2, Glucose Transporter Type 1, medicine.diagnostic_test, biology, General Medicine, Middle Aged, Glucose transporters, Type 1 diabetes, Female, Adult, endocrine system, medicine.medical_specialty, Adolescent, Duodenum, 030209 endocrinology & metabolism, Immunofluorescence, Young Adult, 03 medical and health sciences, Sodium-Glucose Transporter 1, Sodium-Glucose Transporter 2, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Animals, Humans, Aged, business.industry, Glucose transporter, nutritional and metabolic diseases, medicine.disease, Diabetes Mellitus, Type 1, Diabetes Mellitus, Type 2, Gut mucosa, biology.protein, GLUT2, GLUT1, business |
| الوصف: | Aims: A higher SGLT1 and GLUT2 gene expression was shown in the intestine of subjects with type 2 diabetes, while no data have been reported in type 1 diabetes (T1D). The purpose of our study was to evaluate the expression of glucose transporters in duodenal mucosa of subjects with T1D, compared to healthy controls (CTRL) and to patients with celiac disease (CD), as gut inflammatory disease control group. Materials and methods: Gene expression of GLUT1, GLUT2, SGLT1 and SGLT2 was quantified on duodenal mucosa biopsies of subjects with T1D (n = 19), CD (n = 16), T1D and CD (n = 6) and CTRL (n = 12), recruited at San Raffaele Hospital (Milan, Italy), between 2009 and 2018. SGLT2 expression was further evaluated by immunohistochemical and immunofluorescence staining. Results: The expression of all four glucose transporters was detected in duodenal mucosa of all groups. A reduced GLUT2, SGLT1 and SGLT2 expression was observed in CD in comparison with T1D and CTRL, as expected; GLUT1 was significantly more expressed in T1D compared to CTRL. SGLT2 expression was quantified at much lower levels than other transporters, with no differences between groups. SGLT2 expression was confirmed by immunohistochemistry in a restricted number of enterocytes lining in the mucosa of intestinal villi, also shown on immunofluorescence. Conclusions: Our results show that glucose transporters expression in duodenal mucosa of subjects with T1D, except an increased GLUT1, is not different from that observed in healthy controls. The expression of SGLT2 in human duodenal mucosa, although at low intensity, represents a novel finding. |
| تدمد: | 1432-5233 0940-5429 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e27718340c1166c81e866eae31453b0Test https://doi.org/10.1007/s00592-020-01558-wTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2e27718340c1166c81e866eae31453b0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14325233 09405429 |
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