دورية أكاديمية

Four temporin-derived peptides exhibit antimicrobial and antibiofilm activities against methicillin-resistant Staphylococcus aureus

التفاصيل البيبلوغرافية
العنوان: Four temporin-derived peptides exhibit antimicrobial and antibiofilm activities against methicillin-resistant Staphylococcus aureus
المؤلفون: Tan Xiuchuan, Xie Shenghong, Jin Xiao, Zhu Ruiying, Wei Shuangshuang, Song Yanting, Xie Xi, Wang Rong, Li Lushuang, Wang Manchuriga, Zhang Yingxia
المصدر: Acta Biochimica et Biophysica Sinica, Vol 54, Pp 350-360 (2022)
بيانات النشر: China Science Publishing & Media Ltd., 2022.
سنة النشر: 2022
المجموعة: LCC:Biochemistry
LCC:Genetics
مصطلحات موضوعية: antimicrobial peptide, antibacterial activity, antibiofilm activity, methicillin-resistant Staphylococcus aureus, Biochemistry, QD415-436, Genetics, QH426-470
الوصف: Temporin-GHa (GHa) was cloned from Hylarana guentheri, showing a weak antimicrobial activity. In order to improve its bactericidal efficacy, GHaR6R, GHaR7R, GHaR8R and GHaR9W were designed and synthesized. Compared to the parent peptide, the GHa-derived peptides show potent antimicrobial activities against methicillin-resistant Staphylococcus aureus (MRSA), which is the main pathogen with high morbidity and mortality that causes various infections in humans. These peptides exert bactericidal actions on MRSA by permeabilizing the cytoplasmic membranes and damaging membrane integrity. All of the four peptides exhibit excellent stability under harsh conditions, including extreme temperature and salts. Furthermore, they inhibit the formation of biofilm and eradicate mature biofilm of MRSA. The GHa-derived peptides decrease bacterial surface hydrophobicity, autoaggregation and polysaccharide intercellular adhesion synthesis in concentration-dependent manner. Real-time quantitative reverse transcription PCR analysis revealed that the peptides downregulate the expression of adhesion genes icaADBC involved in biofilm formation. Except for GHaR7R, the other three peptides have low hemolytic toxicity against human erythrocytes. In the presence of human erythrocytes, GHaR7R, GHaR8R and GHaR9W interact with MRSA preferentially. GHaR6R, GHaR8R and GHaR9W show less toxicity toward normal cells HL-7702 and hFOB1.19. These results suggest that the GHa-derived peptides may be promising antimicrobial candidates against MRSA infections.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1672-9145
العلاقة: https://doaj.org/toc/1672-9145Test
DOI: 10.3724/abbs.2022013
الوصول الحر: https://doaj.org/article/2027339a1ab54c67a47664ba591edf72Test
رقم الانضمام: edsdoj.2027339a1ab54c67a47664ba591edf72
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:16729145
DOI:10.3724/abbs.2022013