دورية أكاديمية
A Far-Red Fluorescent Probe to Visualize Gram-Positive Bacteria in Patient Samples.
| العنوان: | A Far-Red Fluorescent Probe to Visualize Gram-Positive Bacteria in Patient Samples. |
|---|---|
| المؤلفون: | Jantarug, Krittapas, Tripathi, Vishwachi, Morin, Benedict, Iizuka, Aya, Kuehl, Richard, Morgenstern, Mario, Clauss, Martin, Khanna, Nina, Bumann, Dirk, Rivera-Fuentes, Pablo |
| المصدر: | ACS Infect Dis ; ISSN:2373-8227 ; Volume:10 ; Issue:5 |
| بيانات النشر: | American Chemical Society |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | Gram-positive, S. aureus, fluorescent probe, imaging, super-resolution microscopy, vancomycin |
| الوصف: | Gram-positive bacteria, in particular Staphylococcus aureus (S. aureus), are the leading bacterial cause of death in high-income countries and can cause invasive infections at various body sites. These infections are associated with prolonged hospital stays, a large economic burden, considerable treatment failure, and high mortality rates. So far, there is only limited knowledge about the specific locations where S. aureus resides in the human body during various infections. Hence, the visualization of S. aureus holds significant importance in microbiological research. Herein, we report the development and validation of a far-red fluorescent probe to detect Gram-positive bacteria, with a focus on staphylococci, in human biopsies from deep-seated infections. This probe displays strong fluorescence and low background in human tissues, outperforming current tools for S. aureus detection. Several applications are demonstrated, including fixed- and live-cell imaging, flow cytometry, and super-resolution bacterial imaging. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1021/acsinfecdis.4c00060Test; https://pubmed.ncbi.nlm.nih.gov/38632685Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11091877Test/ |
| DOI: | 10.1021/acsinfecdis.4c00060 |
| الإتاحة: | https://doi.org/10.1021/acsinfecdis.4c00060Test https://pubmed.ncbi.nlm.nih.gov/38632685Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11091877Test/ |
| رقم الانضمام: | edsbas.7BBF77F3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acsinfecdis.4c00060 |
|---|