Topically Applied Chitosan-Coated Poly(isobutylcyanoacrylate) Nanoparticles Are Active Against Cutaneous Leishmaniasis by Accelerating Lesion Healing and Reducing the Parasitic Load
| العنوان: | Topically Applied Chitosan-Coated Poly(isobutylcyanoacrylate) Nanoparticles Are Active Against Cutaneous Leishmaniasis by Accelerating Lesion Healing and Reducing the Parasitic Load |
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| المؤلفون: | Kawthar Bouchemal, Philippe M. Loiseau, Antonio Da Costa, Yasmine Ayadi, Sébastien Pomel, Sophia Malli, Claudine Deloménie |
| المساهمون: | Institut Galien Paris-Sud (IGPS), Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Biomolécules : Conception, Isolement, Synthèse (BioCIS), Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-CY Cergy Paris Université (CY), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-CY Cergy Paris Université (CY), Institut Paris Saclay d’Innovation Thérapeutique (IPSIT), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ACS Applied Bio Materials ACS Applied Bio Materials, 2019, ACS Applied Bio Materials, 2 (6), pp.2573-2586. ⟨10.1021/acsabm.9b00263⟩ |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Biomedical Engineering, Pharmacology, Biomaterials, Chitosan, Lesion, chemistry.chemical_compound, Cutaneous leishmaniasis, In vivo, medicine, Leishmania major, Amastigote, leishmaniasis, biology, Biochemistry (medical), Leishmaniasis, [CHIM.CATA]Chemical Sciences/Catalysis, General Chemistry, medicine.disease, biology.organism_classification, In vitro, chemistry, nanoparticles, chitosan, medicine.symptom, poly(isobutylcyanoacrylate) |
| الوصف: | International audience; Parenteral administration of amphotericin B-deoxycholate (AmB-DOC) or pentavalent antimonials to cure cutaneous leishmaniasis (CL) results in severe adverse reactions, while topically applied antileishmanial drugs are ineffective despite their good tolerance. This work is aimed to investigate whether poly(isobutylcyanoacrylate) nanoparticles coated with chitosan (Cs-NPs) could provide intrinsic antileishmanial activity after topical application. In vitro evaluations revealed that nanoparticles were active against the promastigote, axenic amastigote, and intramacrophage forms of Leishmania major. In vivo evaluations after repetitive topical applications on the skin of mice infected with L. major showed that Cs-NPs combined or not with AmB-DOC allowed partial healing of the lesion characterized by histological analyses. The parasitic load of skin specimens collected from mice was significantly reduced compared with that from nontreated mice, as analyzed by quantitative polymerase chain reaction (q-PCR). Ultrastructure characterizations by electron microscopy of L. major promastigotes after incubation with Cs-NPs showed morphological alterations, including aberrant shape and swelling of mitochondria and parasitic vacuoles. |
| تدمد: | 2576-6422 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66da784c65342aaf11eacbdfa7a37094Test https://doi.org/10.1021/acsabm.9b00263Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....66da784c65342aaf11eacbdfa7a37094 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25766422 |
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