In this paper, a label free approach of the DNA hybridization detection by the guided-mode resonance (GMR) spectroscopy sensor is presented. The GMR biosensor can easily detect any slight variation of the DNA hybridization by the resonant wavelength peak shift. The corresponding peak wavelength shifts of the capture DNA, the target DNA and the probe DNA serially hybridized reactions from GMR are measured, respectively. Furthermore, the stability and reliability of the GMR biosensor for nucleic acid hybridization have also been examined. The result obviously reveals the feasibility of implementation of the GMR-based DNA microarray for detecting on the dynamics of DNA hybridization.
