دورية أكاديمية
Characterisation of emergent toxigenic M1UK Streptococcus pyogenes and associated sublineages
| العنوان: | Characterisation of emergent toxigenic M1UK Streptococcus pyogenes and associated sublineages |
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| المؤلفون: | Li, H-K, Zhi, X, Vieira, A, Whitwell, HJ, Schricker, A, Jauneikaite, E, Li, H, Yosef, A, Andrew, I, Game, L, Turner, CE, Lamagni, T, Coelho, J, Sriskandan, S |
| المصدر: | 15 ; 1 |
| بيانات النشر: | Microbiology Society |
| سنة النشر: | 2023 |
| المجموعة: | Imperial College London: Spiral |
| الوصف: | Streptococcus pyogenes genotype emm1 is a successful, globally distributed epidemic clone that is regarded as inherently virulent. An emm1 sublineage, M1UK, that produces increased levels of SpeA toxin was associated with increased scarlet fever and invasive infections in England in 2015/2016. Defined by 27 SNPs in the core genome, M1UK is now dominant in England. To more fully characterize M1UK, we undertook comparative transcriptomic and proteomic analyses of M1UK and contemporary non-M1UKemm1 strains (M1global). Just seven genes were differentially expressed by M1UK compared with contemporary M1global strains. In addition to speA, five genes in the operon that includes glycerol dehydrogenase were upregulated in M1UK (gldA, mipB/talC, pflD, and phosphotransferase system IIC and IIB components), while aquaporin (glpF2) was downregulated. M1UK strains have a stop codon in gldA. Deletion of gldA in M1global abrogated glycerol dehydrogenase activity, and recapitulated upregulation of gene expression within the operon that includes gldA, consistent with a feedback effect. Phylogenetic analysis identified two intermediate emm1 sublineages in England comprising 13/27 (M113SNPs) and 23/27 SNPs (M123SNPs), respectively, that had failed to expand in the population. Proteomic analysis of invasive strains from the four phylogenetic emm1 groups highlighted sublineage-specific changes in carbohydrate metabolism, protein synthesis and protein processing; upregulation of SpeA was not observed in chemically defined medium. In rich broth, however, expression of SpeA was upregulated ~10-fold in both M123SNPs and M1UK sublineages, compared with M113SNPs and M1global. We conclude that stepwise accumulation of SNPs led to the emergence of M1UK. While increased expression of SpeA is a key indicator of M1UK and undoubtedly important, M1UK strains have outcompeted M123SNPs and other emm types that produce similar or more superantigen toxin. We speculate that an accumulation of adaptive SNPs has contributed to a wider fitness advantage ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 2057-5858 |
| العلاقة: | Microbial Genomics; http://hdl.handle.net/10044/1/103489Test |
| DOI: | 10.1099/mgen.0.000994 |
| الإتاحة: | https://doi.org/10.1099/mgen.0.000994Test http://hdl.handle.net/10044/1/103489Test |
| حقوق: | © The Author(s), 2023. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.22417D55 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20575858 |
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| DOI: | 10.1099/mgen.0.000994 |