دورية أكاديمية
HLA Class I Upregulation and Antiviral Immune Responses in Graves Disease
| العنوان: | HLA Class I Upregulation and Antiviral Immune Responses in Graves Disease |
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| المؤلفون: | Weider, Therese, Richardson, Sarah J., Morgan, Noel G., Paulsen, Trond Harder, Dahl-Jørgensen, Knut, Hammerstad, Sara Salehi |
| المصدر: | 0021-972X. |
| سنة النشر: | 2021 |
| المجموعة: | Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
| الوصف: | Context The origin of Graves disease (GD) remains elusive. However, evidence of an association between GD and viral infections is emerging. Human leukocyte antigen (HLA) class I presents viral antigens to circulating immune cells and plays a crucial role in the defense against viral infections. Objective This work aimed to investigate HLA class I expression, enterovirus presence, and the viral immune response proteins signal transducer and activation of transcription 1 (STAT1) and protein kinase R (PKR) in thyroid tissue from GD patients. Methods We collected thyroid tissue from core needle biopsies or surgical specimens from 48 GD patients and 24 controls. Standard immunohistochemistry was used to detect HLA class I and enteroviral capsid protein 1 (VP1) on formalin-fixed and paraffin-embedded tissue. STAT1 and PKR were examined by combined immunofluorescence staining. HLA class I expression score was the main outcome measure. Results The HLA class I expression score, which takes both proportion and intensity of immunostaining into account, was significantly higher in GD patients (3.1 ± 3.3) than in controls (0.5 ± 0.9) (P < .001). Significantly more VP1 positive thyroid cells were found GD samples (50.1 ± 30.5%) than in controls (14.9 ± 10.5%) (P < .001). STAT1 and HLA class I were found within the same thyroid cells and PKR and VP1 were also colocalized within thyroid cells. Conclusion HLA class I is upregulated in GD and enterovirus protein is prevalent in thyroid tissue. The colocalization of HLA class I with STAT1 and VP1 with PKR indicates an antiviral tissue response. These findings support the concept of a link between viral infections and GD. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://urn.nb.no/URN:NBN:no-94160Test; Weider, Therese Richardson, Sarah J. Morgan, Noel G. Paulsen, Trond Harder Dahl-Jørgensen, Knut Hammerstad, Sara Salehi . HLA Class I Upregulation and Antiviral Immune Responses in Graves Disease. Journal of Clinical Endocrinology and Metabolism (JCEM). 2021, 106(4), 1763-1774; http://hdl.handle.net/10852/91569Test; 1920740; info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of Clinical Endocrinology and Metabolism (JCEM)&rft.volume=106&rft.spage=1763&rft.date=2021; Journal of Clinical Endocrinology and Metabolism (JCEM); 106; 1763; 1774; https://doi.org/10.1210/clinem/dgaa958Test; URN:NBN:no-94160; Fulltext https://www.duo.uio.no/bitstream/handle/10852/91569/1/Weider_et_al_2021.pdfTest |
| DOI: | 10.1210/clinem/dgaa958 |
| الإتاحة: | https://doi.org/10.1210/clinem/dgaa958Test http://hdl.handle.net/10852/91569Test http://urn.nb.no/URN:NBN:no-94160Test |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 International ; https://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.41550AFB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1210/clinem/dgaa958 |
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