دورية أكاديمية
Whole blood methylome-derived features to discriminate endocrine hypertension
| العنوان: | Whole blood methylome-derived features to discriminate endocrine hypertension |
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| المؤلفون: | Armignacco, Roberta, Reel, Parminder S., Reel, Smarti, Jouinot, Anne, Septier, Amandine, Gaspar, Cassandra, Perlemoine, Karine, Larsen, Casper K., Bouys, Lucas, Braun, Leah, Riester, Anna, Kroiss, Matthias, Bonnet-Serrano, Fidéline, Amar, Laurence, Blanchard, Anne, Gimenez-Roqueplo, Anne-Paule, Prejbisz, Aleksander, Januszewicz, Andrzej, Dobrowolski, Piotr, Davies, Eleanor, MacKenzie, Scott M., Rossi, Gian Paolo, Lenzini, Livia, Ceccato, Filippo, Scaroni, Carla, Mulatero, Paolo, Williams, Tracy A, Pecori, Alessio, Monticone, Silvia, Beuschlein, Felix, Reincke, Martin, Zennaro, Maria-Christina, Bertherat, Jérôme, Jefferson, Emily, Assié, Guillaume |
| المصدر: | Armignacco , R , Reel , P S , Reel , S , Jouinot , A , Septier , A , Gaspar , C , Perlemoine , K , Larsen , C K , Bouys , L , Braun , L , Riester , A , Kroiss , M , Bonnet-Serrano , F , Amar , L , Blanchard , A , Gimenez-Roqueplo , A-P , Prejbisz , A , Januszewicz , A , Dobrowolski , P , Davies , E , MacKenzie , S M , Rossi , G P , Lenzini , L , Ceccato , F .... |
| سنة النشر: | 2022 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | Humans, Epigenome, DNA Methylation, Pheochromocytoma/complications, Hypertension/diagnosis, Adrenal Gland Neoplasms/diagnosis, Biomarkers, Circulating biomarker, Whole blood methylome, Endocrine hypertension, /dk/atira/pure/subjectarea/asjc/2700/2716, name=Genetics(clinical), /dk/atira/pure/subjectarea/asjc/1300/1311, name=Genetics, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1309, name=Developmental Biology |
| الوصف: | Background : Arterial hypertension represents a worldwide health burden and a major risk factor for cardiovascular morbidity and mortality. Hypertension can be primary (primary hypertension, PHT), or secondary to endocrine disorders (endocrine hypertension, EHT), such as Cushing's syndrome (CS), primary aldosteronism (PA), and pheochromocytoma/paraganglioma (PPGL). Diagnosis of EHT is currently based on hormone assays. Efficient detection remains challenging, but is crucial to properly orientate patients for diagnostic confirmation and specific treatment. More accurate biomarkers would help in the diagnostic pathway. We hypothesized that each type of endocrine hypertension could be associated with a specific blood DNA methylation signature, which could be used for disease discrimination. To identify such markers, we aimed at exploring the methylome profiles in a cohort of 255 patients with hypertension, either PHT (n = 42) or EHT (n = 213), and at identifying specific discriminating signatures using machine learning approaches. Results : Unsupervised classification of samples showed discrimination of PHT from EHT. CS patients clustered separately from all other patients, whereas PA and PPGL showed an overall overlap. Global methylation was decreased in the CS group compared to PHT. Supervised comparison with PHT identified differentially methylated CpG sites for each type of endocrine hypertension, showing a diffuse genomic location. Among the most differentially methylated genes, FKBP5 was identified in the CS group. Using four different machine learning methods-Lasso (Least Absolute Shrinkage and Selection Operator), Logistic Regression, Random Forest, and Support Vector Machine-predictive models for each type of endocrine hypertension were built on training cohorts (80% of samples for each hypertension type) and estimated on validation cohorts (20% of samples for each hypertension type). Balanced accuracies ranged from 0.55 to 0.74 for predicting EHT, 0.85 to 0.95 for predicting CS, 0.66 to 0.88 for ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/0cfe44a3-d660-4363-8376-a8a0cf3d731bTest |
| DOI: | 10.1186/s13148-022-01347-y |
| الإتاحة: | https://doi.org/10.1186/s13148-022-01347-yTest https://discovery.dundee.ac.uk/en/publications/0cfe44a3-d660-4363-8376-a8a0cf3d731bTest https://discovery.dundee.ac.uk/ws/files/88193183/s13148_022_01347_y.pdfTest http://www.scopus.com/inward/record.url?scp=85141171757&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D7565ED3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13148-022-01347-y |
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