المؤلفون: Redó-Riveiro, Alba, Al-Mousawi, Jasmina, Linneberg-Agerholm, Madeleine, Proks, Martin, Perera, Marta, Salehin, Nazmus, Brickman, Joshua M.

المصدر: Redó-Riveiro , A , Al-Mousawi , J , Linneberg-Agerholm , M , Proks , M , Perera , M , Salehin , N & Brickman , J M 2024 , ' Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation ' , Stem Cell Reports , vol. 19 , no. 2 , pp. 174-186 . https://doi.org/10.1016/j.stemcr.2023.12.002Test

مصطلحات موضوعية: Blastocyst, Cooperativity, Embryonic Stem Cells, Endoderm, Enhancer, Epiblast, Lineage Priming, nEnd, Pluripotency, Transcription

الوصف: In early mammalian development, cleavage stage blastomeres and inner cell mass (ICM) cells co-express embryonic and extra-embryonic transcriptional determinants. Using a protein-based double reporter we identify an embryonic stem cell (ESC) population that co-expresses the extra-embryonic factor GATA6 alongside the embryonic factor SOX2. Based on single cell transcriptomics, we find this population resembles the unsegregated ICM, exhibiting enhanced differentiation potential for endoderm while maintaining epiblast competence. To relate transcription factor binding in these cells to future fate, we describe a complete enhancer set in both ESCs and naive extra-embryonic endoderm stem cells and assess SOX2 and GATA6 binding at these elements in the ICM-like ESC sub-population. Both factors support cooperative recognition in these lineages, with GATA6 bound alongside SOX2 on a fraction of pluripotency enhancers and SOX2 alongside GATA6 more extensively on endoderm enhancers, suggesting that cooperative binding between these antagonistic factors both supports self-renewal and prepares progenitor cells for later differentiation. ; In early mammalian development, cleavage stage blastomeres and inner cell mass (ICM) cells co-express embryonic and extra-embryonic transcriptional determinants. Using a protein-based double reporter we identify an embryonic stem cell (ESC) population that co-expresses the extra-embryonic factor GATA6 alongside the embryonic factor SOX2. Based on single cell transcriptomics, we find this population resembles the unsegregated ICM, exhibiting enhanced differentiation potential for endoderm while maintaining epiblast competence. To relate transcription factor binding in these cells to future fate, we describe a complete enhancer set in both ESCs and naive extra-embryonic endoderm stem cells and assess SOX2 and GATA6 binding at these elements in the ICM-like ESC sub-population. Both factors support cooperative recognition in these lineages, with GATA6 bound alongside SOX2 on a fraction of ...

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1016/j.stemcr.2023.12.002Test
https://curis.ku.dk/portal/da/publications/transcription-factor-coexpression-mediates-lineage-priming-for-embryonic-and-extraembryonic-differentiationTest(e1ec4898-aa0f-4b33-8787-d317e5f9afb1).html
https://curis.ku.dk/ws/files/382985721/1_s2.0_S2213671123004952_main.pdfTest

المؤلفون: Linneberg-Agerholm, Madeleine, Brickman, Joshua Mark

المصدر: Linneberg-Agerholm , M & Brickman , J M 2022 , Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem Cells . in Methods in Molecular Biology . Humana Press , Methods in Molecular Biology , vol. 2416 , pp. 105-116 . https://doi.org/10.1007/978-1-0716-1908-7_8Test

مصطلحات موضوعية: Embryonic stem cells, Endoderm, Extra-embryonic, Human, Hypoblast, Naïve, Pluripotency, Primitive endoderm

الوصف: In human, endoderm is induced in two waves, with the first being the extra-embryonic primitive endoderm (PrE), otherwise known as hypoblast, induced during blastocyst development, and the second being gastrulation-stage definitive endoderm (DE). The PrE gives rise to the primary and secondary yolk sac, and has supportive functions during pregnancy for nutrient provision, with descendants of this extra-embryonic lineage also playing a role in embryonic patterning. As in DE specification, we recently found that PrE could be induced in vitro by Wnt and Nodal-related signaling, but that the critical difference was in the pluripotent starting point for differentiation. Thus, blastocyst-like naïve human pluripotent stem cells retain the unique capacity to differentiate into PrE cultures, a cell type resembling the pre-implantation hypoblast. The PrE cells could then be expanded as stable naïve extra-embryonic endoderm (nEnd) cell lines, capable of indefinite self-renewal. Here, we describe detailed protocols to differentiate naïve pluripotent stem cells into PrE and then expand the cultures as nEnd, including descriptions of morphology, passaging technique, and troubleshooting.

الإتاحة: https://doi.org/10.1007/978-1-0716-1908-7_8Test
https://curis.ku.dk/portal/da/publications/differentiation-and-expansion-of-human-extraembryonicTest-endoderm-cell-lines-from-naive-pluripotent-stem-cells(30910c10-f6a8-4016-ae5e-2684f2ee97f2).html

المؤلفون: Mitchell, Adam, Yu, Chaowen, Zhao, Xiangjun, Pearmain, Laurence, Shah, Rajesh, Hanley, Karen Piper, Felton, Timothy, Wang, Tao

المصدر: Mitchell , A , Yu , C , Zhao , X , Pearmain , L , Shah , R , Hanley , K P , Felton , T & Wang , T 2023 , ' Rapid Generation of Pulmonary Organoids from Induced Pluripotent Stem Cells by Co-Culturing Endodermal and Mesodermal Progenitors for Pulmonary Disease Modelling ' , Biomedicines , vol. 11 , no. 5 , 1476 . https://doi.org/10.3390/biomedicines11051476Test

مصطلحات موضوعية: pulmonary organoids, induced pluripotent stem cells (iPSCs), anterior foregut endoderm, mesoderm, alveoli epithelial cells, SARS-CoV-2, iPSC disease modelling

الوصف: Differentiation of induced pluripotent stem cells to a range of target cell types is ubiquitous in monolayer culture. To further improve the phenotype of the cells produced, 3D organoid culture is becoming increasingly prevalent. Mature organoids typically require the involvement of cells from multiple germ layers. The aim of this study was to produce pulmonary organoids from defined endodermal and mesodermal progenitors. Endodermal and mesodermal progenitors were differentiated from iPSCs and then combined in 3D Matrigel hydrogels and differentiated for a further 14 days to produce pulmonary organoids. The organoids expressed a range of pulmonary cell markers such as SPA, SPB, SPC, AQP5 and T1α. Furthermore, the organoids expressed ACE2 capable of binding SARS-CoV-2 spike proteins, demonstrating the physiological relevance of the organoids produced. This study presented a rapid production of pulmonary organoids using a multi-germ-layer approach that could be used for studying respiratory-related human conditions.

وصف الملف: application/pdf

العلاقة: https://research.manchester.ac.uk/en/publications/9a1535e2-30ce-4427-8cb2-4f0a61b5e77bTest

الإتاحة: https://doi.org/10.3390/biomedicines11051476Test
https://research.manchester.ac.uk/en/publications/9a1535e2-30ce-4427-8cb2-4f0a61b5e77bTest
https://pure.manchester.ac.uk/ws/files/263159921/biomedicines_11_01476_Published_pdf.pdfTest

المؤلفون: Farkas, Karin, Ferretti, Elisabetta

المصدر: Farkas , K & Ferretti , E 2023 , ' Derivation of Human Extraembryonic Mesoderm-like Cells from Primitive Endoderm ' , International Journal of Molecular Sciences , vol. 24 , no. 14 , 11366 . https://doi.org/10.3390/ijms241411366Test

مصطلحات موضوعية: extraembryonic mesoderm, human gastrulation, hypoblast, placenta, primitive endoderm, vasculogenesis, yolk sac

الوصف: In vitro modeling of human peri-gastrulation development is a valuable tool for understanding embryogenetic mechanisms. The extraembryonic mesoderm (ExM) is crucial in supporting embryonic development by forming tissues such as the yolk sac, allantois, and chorionic villi. However, the origin of human ExM remains only partially understood. While evidence suggests a primitive endoderm (PrE) origin based on morphological findings, current in vitro models use epiblast-like cells. To address this gap, we developed a protocol to generate ExM-like cells from PrE-like cell line called naïve extraembryonic endoderm (nEnd). We identified the ExM-like cells by specific markers (LUM and ANXA1). Moreover, these in vitro-produced ExM cells displayed angiogenic potential on a soft matrix, mirroring their physiological role in vasculogenesis. By integrating single-cell RNA sequencing (scRNAseq) data, we found that the ExM-like cells clustered with the LUM/ANXA1-rich cell populations of the gastrulating embryo, indicating similarity between in vitro and ex utero cell populations. This study confirms the derivation of ExM from PrE and establishes a cell culture system that can be utilized to investigate ExM during human peri-gastrulation development, both in monolayer cultures and more complex models. ; In vitro modeling of human peri-gastrulation development is a valuable tool for understanding embryogenetic mechanisms. The extraembryonic mesoderm (ExM) is crucial in supporting embryonic development by forming tissues such as the yolk sac, allantois, and chorionic villi. However, the origin of human ExM remains only partially understood. While evidence suggests a primitive endoderm (PrE) origin based on morphological findings, current in vitro models use epiblast-like cells. To address this gap, we developed a protocol to generate ExM-like cells from PrE-like cell line called naïve extraembryonic endoderm (nEnd). We identified the ExM-like cells by specific markers (LUM and ANXA1). Moreover, these in vitro-produced ExM cells ...

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.3390/ijms241411366Test
https://curis.ku.dk/portal/da/publications/derivation-of-human-extraembryonic-mesodermlike-cells-from-primitiveTest-endoderm(bde3ac16-a571-41db-a7ac-904673bbc261).html
https://curis.ku.dk/ws/files/361590471/ijms_24_11366.pdfTest

المؤلفون: Pistollato, Francesca, Bal-Price, Anna, Coecke, Sandra, Parvatam, Surat, Pamies, David, Czysz, Katherine, Hao, Jie, Kee, Kehkooi, Teo, Adrian Kee Keong, Niu, Shuaishuai, Wilmes, Anja, Smirnova, Lena, Freund, Christian, Mummery, Christine, Stacey, Glyn

المصدر: Pistollato , F , Bal-Price , A , Coecke , S , Parvatam , S , Pamies , D , Czysz , K , Hao , J , Kee , K , Teo , A K K , Niu , S , Wilmes , A , Smirnova , L , Freund , C , Mummery , C & Stacey , G 2022 , ' Quality criteria for in vitro human pluripotent stem cell-derived models of tissue-based cells ' , Reproductive Toxicology , vol. 112 , pp. 36-50 . https://doi.org/10.1016/j.reprotox.2022.06.003Test

مصطلحات موضوعية: 3D, Differentiation, Ectoderm, Endoderm, GCCP, Human pluripotent stem cells, In vitro toxicology, iPSCs, Mesoderm, Quality control criteria, Readiness level, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being

الوصف: The advent of the technology to isolate or generate human pluripotent stem cells provided the potential to develop a wide range of human models that could enhance understanding of mechanisms underlying human development and disease. These systems are now beginning to mature and provide the basis for the development of in vitro assays suitable to understand the biological processes involved in the multi-organ systems of the human body, and will improve strategies for diagnosis, prevention, therapies and precision medicine. Induced pluripotent stem cell lines are prone to phenotypic and genotypic changes and donor/clone dependent variability, which means that it is important to identify the most appropriate characterization markers and quality control measures when sourcing new cell lines and assessing differentiated cell and tissue culture preparations for experimental work. This paper considers those core quality control measures for human pluripotent stem cell lines and evaluates the state of play in the development of key functional markers for their differentiated cell derivatives to promote assurance of reproducibility of scientific data derived from pluripotent stem cell-based systems.

وصف الملف: application/pdf

العلاقة: https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test

الإتاحة: https://doi.org/10.1016/j.reprotox.2022.06.003Test
https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://hdl.handle.net/1871.1/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://research.vu.nl/ws/files/170377697/Quality_criteria_for_in_vitro_human_pluripotent_stem_cell_derived_models_of_tissue_based_cells.pdfTest
http://www.scopus.com/inward/record.url?scp=85133253702&partnerID=8YFLogxKTest
http://www.scopus.com/inward/citedby.url?scp=85133253702&partnerID=8YFLogxKTest

المؤلفون: Pistollato, Francesca, Bal-Price, Anna, Coecke, Sandra, Parvatam, Surat, Pamies, David, Czysz, Katherine, Hao, Jie, Kee, Kehkooi, Teo, Adrian Kee Keong, Niu, Shuaishuai, Wilmes, Anja, Smirnova, Lena, Freund, Christian, Mummery, Christine, Stacey, Glyn

المصدر: Pistollato , F , Bal-Price , A , Coecke , S , Parvatam , S , Pamies , D , Czysz , K , Hao , J , Kee , K , Teo , A K K , Niu , S , Wilmes , A , Smirnova , L , Freund , C , Mummery , C & Stacey , G 2022 , ' Quality criteria for in vitro human pluripotent stem cell-derived models of tissue-based cells ' , Reproductive Toxicology , vol. 112 , pp. 36-50 . https://doi.org/10.1016/j.reprotox.2022.06.003Test

مصطلحات موضوعية: 3D, Differentiation, Ectoderm, Endoderm, GCCP, Human pluripotent stem cells, In vitro toxicology, iPSCs, Mesoderm, Quality control criteria, Readiness level, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being

الوصف: The advent of the technology to isolate or generate human pluripotent stem cells provided the potential to develop a wide range of human models that could enhance understanding of mechanisms underlying human development and disease. These systems are now beginning to mature and provide the basis for the development of in vitro assays suitable to understand the biological processes involved in the multi-organ systems of the human body, and will improve strategies for diagnosis, prevention, therapies and precision medicine. Induced pluripotent stem cell lines are prone to phenotypic and genotypic changes and donor/clone dependent variability, which means that it is important to identify the most appropriate characterization markers and quality control measures when sourcing new cell lines and assessing differentiated cell and tissue culture preparations for experimental work. This paper considers those core quality control measures for human pluripotent stem cell lines and evaluates the state of play in the development of key functional markers for their differentiated cell derivatives to promote assurance of reproducibility of scientific data derived from pluripotent stem cell-based systems.

وصف الملف: application/pdf

العلاقة: https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test

الإتاحة: https://doi.org/10.1016/j.reprotox.2022.06.003Test
https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://hdl.handle.net/1871.1/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://research.vu.nl/ws/files/170377697/Quality_criteria_for_in_vitro_human_pluripotent_stem_cell_derived_models_of_tissue_based_cells.pdfTest
http://www.scopus.com/inward/record.url?scp=85133253702&partnerID=8YFLogxKTest
http://www.scopus.com/inward/citedby.url?scp=85133253702&partnerID=8YFLogxKTest

المؤلفون: Vrij, Erik J, Scholte Op Reimer, Yvonne S, Fuentes, Laury Roa, Guerreiro, Isabel Misteli, Holzmann, Viktoria, Aldeguer, Javier Frias, Sestini, Giovanni, Koo, Bon-Kyoung, Kind, Jop, van Blitterswijk, Clemens A, Rivron, Nicolas C

المصدر: Vrij , E J , Scholte Op Reimer , Y S , Fuentes , L R , Guerreiro , I M , Holzmann , V , Aldeguer , J F , Sestini , G , Koo , B-K , Kind , J , van Blitterswijk , C A & Rivron , N C 2022 , ' A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression ' , Development , vol. 149 , no. 20 , dev192310 . https://doi.org/10.1242/dev.192310Test

مصطلحات موضوعية: Animals, Blastocyst, Cell Differentiation, Cell Lineage/physiology, Embryo Implantation, Embryo, Mammalian, Endoderm, Germ Layers, Mice

الوصف: Embryogenesis is supported by dynamic loops of cellular interactions. Here, we create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn). We trigger naive mouse embryonic stem cells to form a blastocyst-stage niche of Epi-like cells and XEn-like cells (3D, hydrogel free and serum free). Once established, these two lineages autonomously progress in minimal medium to form an inner pro-amniotic-like cavity surrounded by polarized Epi-like cells covered with visceral endoderm (VE)-like cells. The progression occurs through reciprocal inductions by which the Epi supports the primitive endoderm (PrE) to produce a basal lamina that subsequently regulates Epi polarization and/or cavitation, which, in return, channels the transcriptomic progression to VE. This VE then contributes to Epi bifurcation into anterior- and posterior-like states. Similarly, boosting the formation of PrE-like cells within blastoids supports developmental progression. We argue that self-organization can arise from lineage bifurcation followed by a pendulum of induction that propagates over time.

العلاقة: https://cris.maastrichtuniversity.nl/en/publications/9d260439-56bc-4d04-bc18-acd9e89750d1Test

الإتاحة: https://doi.org/10.1242/dev.192310Test
https://cris.maastrichtuniversity.nl/en/publications/9d260439-56bc-4d04-bc18-acd9e89750d1Test

المؤلفون: Luijkx, Dorian, Shankar, Vinidhra, van Blitterswijk, Clemens, Giselbrecht, Stefan, Vrij, Erik

المصدر: Luijkx , D , Shankar , V , van Blitterswijk , C , Giselbrecht , S & Vrij , E 2022 , ' From Mice to Men : Generation of Human Blastocyst-Like Structures In Vitro ' , Frontiers in Cell and Developmental Biology , vol. 10 , 838356 . https://doi.org/10.3389/fcell.2022.838356Test

مصطلحات موضوعية: blastoids, human blastocyst-like structures, embryonic development, pluripotent stem cells, implantation, EMBRYONIC STEM-CELLS, PRIMITIVE ENDODERM, SELF-ORGANIZATION, MOUSE BLASTOCYSTS, NAIVE PLURIPOTENCY, CULTURE-CONDITIONS, GROUND-STATE, TROPHOBLAST, DERIVATION, MAINTENANCE

الوصف: Advances in the field of stem cell-based models have in recent years lead to the development of blastocyst-like structures termed blastoids. Blastoids can be used to study key events in mammalian pre-implantation development, as they mimic the blastocyst morphologically and transcriptionally, can progress to the post-implantation stage and can be generated in large numbers. Blastoids were originally developed using mouse pluripotent stem cells, and since several groups have successfully generated blastocyst models of the human system. Here we provide a comparison of the mouse and human protocols with the aim of deriving the core requirements for blastoid formation, discuss the models' current ability to mimic blastocysts and give an outlook on potential future applications.

الإتاحة: https://doi.org/10.3389/fcell.2022.838356Test
https://cris.maastrichtuniversity.nl/en/publications/cecc0d58-05ad-4b11-a629-f5fae817c450Test

المؤلفون: Vrij, Erik J, Scholte Op Reimer, Yvonne S, Roa Fuentes, Laury, Misteli Guerreiro, Isabel, Holzmann, Viktoria, Frias Aldeguer, Javier, Sestini, Giovanni, Koo, Bon-Kyoung, Kind, Jop, van Blitterswijk, Clemens A, Rivron, Nicolas C

المصدر: Vrij , E J , Scholte Op Reimer , Y S , Roa Fuentes , L , Misteli Guerreiro , I , Holzmann , V , Frias Aldeguer , J , Sestini , G , Koo , B-K , Kind , J , van Blitterswijk , C A & Rivron , N C 2022 , ' A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression ' , Development (Cambridge) , vol. 149 , no. 20 . https://doi.org/10.1242/dev.192310Test

مصطلحات موضوعية: Animals, Blastocyst, Cell Differentiation, Cell Lineage/physiology, Embryo Implantation, Embryo, Mammalian, Endoderm, Germ Layers, Mice

الوصف: Embryogenesis is supported by dynamic loops of cellular interactions. Here, we create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn). We trigger naive mouse embryonic stem cells to form a blastocyst-stage niche of Epi-like cells and XEn-like cells (3D, hydrogel free and serum free). Once established, these two lineages autonomously progress in minimal medium to form an inner pro-amniotic-like cavity surrounded by polarized Epi-like cells covered with visceral endoderm (VE)-like cells. The progression occurs through reciprocal inductions by which the Epi supports the primitive endoderm (PrE) to produce a basal lamina that subsequently regulates Epi polarization and/or cavitation, which, in return, channels the transcriptomic progression to VE. This VE then contributes to Epi bifurcation into anterior- and posterior-like states. Similarly, boosting the formation of PrE-like cells within blastoids supports developmental progression. We argue that self-organization can arise from lineage bifurcation followed by a pendulum of induction that propagates over time.

العلاقة: https://pure.knaw.nl/portal/en/publications/68d1c94d-eaa5-4672-8b61-3acbd4bcba2dTest

الإتاحة: https://doi.org/10.1242/dev.192310Test
https://doi.org/20.500.11755/68d1c94d-eaa5-4672-8b61-3acbd4bcba2dTest
https://pure.knaw.nl/portal/en/publications/68d1c94d-eaa5-4672-8b61-3acbd4bcba2dTest
https://hdl.handle.net/20.500.11755/68d1c94d-eaa5-4672-8b61-3acbd4bcba2dTest

المؤلفون: Kim, Esther Jeong Yoon, Sorokin, Lydia, Hiiragi, Takashi

المصدر: Kim , E J Y , Sorokin , L & Hiiragi , T 2022 , ' ECM-integrin signalling instructs cellular position sensing to pattern the early mouse embryo ' , Development (Cambridge) , vol. 149 , no. 1 . https://doi.org/10.1242/dev.200140Test

مصطلحات موضوعية: Animals, Body Patterning, Cell Differentiation, Cell Polarity, Cells, Cultured, Ectoderm/cytology, Endoderm/cytology, Extracellular Matrix/metabolism, Integrin beta1/metabolism, Mice, Inbred C57BL, Mouse Embryonic Stem Cells/cytology, Signal Transduction

الوصف: Development entails patterned emergence of diverse cell types within the embryo. In mammals, cells positioned inside the embryo give rise to the inner cell mass (ICM), which eventually forms the embryo itself. Yet, the molecular basis of how these cells recognise their 'inside' position to instruct their fate is unknown. Here, we show that provision of extracellular matrix (ECM) to isolated embryonic cells induces ICM specification and alters the subsequent spatial arrangement between epiblast (EPI) and primitive endoderm (PrE) cells that emerge within the ICM. Notably, this effect is dependent on integrin β1 activity and involves apical-to-basal conversion of cell polarity. We demonstrate that ECM-integrin activity is sufficient for 'inside' positional signalling and is required for correct EPI/PrE patterning. Thus, our findings highlight the significance of ECM-integrin adhesion in enabling position sensing by cells to achieve tissue patterning.

الإتاحة: https://doi.org/10.1242/dev.200140Test
https://doi.org/20.500.11755/f739425a-c5d5-4514-a9f7-0d4dbd80f6c9Test
https://pure.knaw.nl/portal/en/publications/f739425a-c5d5-4514-a9f7-0d4dbd80f6c9Test
https://hdl.handle.net/20.500.11755/f739425a-c5d5-4514-a9f7-0d4dbd80f6c9Test