دورية أكاديمية
A cyclin-dependent kinase-mediated phosphorylation switch of disordered protein condensation
| العنوان: | A cyclin-dependent kinase-mediated phosphorylation switch of disordered protein condensation |
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| المؤلفون: | Valverde, Juan Manuel, Dubra, Geronimo, Phillips, Michael, Haider, Austin, Elena-Real, Carlos, Fournet, Aurélie, Alghoul, Emile, Chahar, Dhanvantri, Andrés-Sanchez, Nuria, Paloni, Matteo, Bernadó, Pau, van Mierlo, Guido, Vermeulen, Michiel, van den Toorn, Henk, Heck, Albert J R, Constantinou, Angelos, Barducci, Alessandro, Ghosh, Kingshuk, Sibille, Nathalie, Knipscheer, Puck, Krasinska, Liliana, Fisher, Daniel, Altelaar, Maarten |
| المصدر: | Valverde , J M , Dubra , G , Phillips , M , Haider , A , Elena-Real , C , Fournet , A , Alghoul , E , Chahar , D , Andrés-Sanchez , N , Paloni , M , Bernadó , P , van Mierlo , G , Vermeulen , M , van den Toorn , H , Heck , A J R , Constantinou , A , Barducci , A , Ghosh , K , Sibille , N , Knipscheer , P , Krasinska , L , Fisher , D & Altelaar , M 2023 , .... |
| سنة النشر: | 2023 |
| المجموعة: | KNAW: Research Explorer (Koninklijke Nederlandse Akademie van Wetenschappen / Royal Netherlands Academy of Arts and Sciences) |
| مصطلحات موضوعية: | Cyclin-Dependent Kinases/metabolism, Phosphorylation, Cell Cycle Proteins/metabolism, Cell Cycle, Cyclin-Dependent Kinase 2/metabolism |
| الوصف: | Cell cycle transitions result from global changes in protein phosphorylation states triggered by cyclin-dependent kinases (CDKs). To understand how this complexity produces an ordered and rapid cellular reorganisation, we generated a high-resolution map of changing phosphosites throughout unperturbed early cell cycles in single Xenopus embryos, derived the emergent principles through systems biology analysis, and tested them by biophysical modelling and biochemical experiments. We found that most dynamic phosphosites share two key characteristics: they occur on highly disordered proteins that localise to membraneless organelles, and are CDK targets. Furthermore, CDK-mediated multisite phosphorylation can switch homotypic interactions of such proteins between favourable and inhibitory modes for biomolecular condensate formation. These results provide insight into the molecular mechanisms and kinetics of mitotic cellular reorganisation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://pure.knaw.nl/portal/en/publications/2108ef2a-e31a-4bba-8abe-934366dacb6eTest |
| DOI: | 10.1038/s41467-023-42049-0 |
| الإتاحة: | https://doi.org/10.1038/s41467-023-42049-0Test https://doi.org/20.500.11755/2108ef2a-e31a-4bba-8abe-934366dacb6eTest https://pure.knaw.nl/portal/en/publications/2108ef2a-e31a-4bba-8abe-934366dacb6eTest https://hdl.handle.net/20.500.11755/2108ef2a-e31a-4bba-8abe-934366dacb6eTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.5B252836 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-42049-0 |
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