المؤلفون: Schäfer, Benedikt, Emonts, Caroline, Glimpel, Nikola, Ruhl, Tim, Obrecht, Astrid S, Jockenhoevel, Stefan, Gries, Thomas, Beier, Justus P, Blaeser, Andreas

المصدر: Schäfer , B , Emonts , C , Glimpel , N , Ruhl , T , Obrecht , A S , Jockenhoevel , S , Gries , T , Beier , J P & Blaeser , A 2020 , ' Warp-Knitted Spacer Fabrics : A Versatile Platform to Generate Fiber-Reinforced Hydrogels for 3D Tissue Engineering ' , Materials , vol. 13 , no. 16 , 3518 . https://doi.org/10.3390/ma13163518Test

مصطلحات موضوعية: tissue engineering, biofabrication, biohybrid scaffold, textile engineering, MESENCHYMAL STEM-CELLS, AUTOLOGOUS BREAST RECONSTRUCTION, HUMAN ADIPOSE-TISSUE, STROMAL CELLS, DIFFERENTIATION, PROLIFERATION, ELECTROSPUN, CONSTRUCTS, PERFUSION, DIEP

الوصف: Mesenchymal stem cells (MSCs) possess huge potential for regenerative medicine. For tissue engineering approaches, scaffolds and hydrogels are routinely used as extracellular matrix (ECM) carriers. The present study investigated the feasibility of using textile-reinforced hydrogels with adjustable porosity and elasticity as a versatile platform for soft tissue engineering. A warp-knitted poly (ethylene terephthalate) (PET) scaffold was developed and characterized with respect to morphology, porosity, and mechanics. The textile carrier was infiltrated with hydrogels and cells resulting in a fiber-reinforced matrix with adjustable biological as well as mechanical cues. Finally, the potential of this platform technology for regenerative medicine was tested on the example of fat tissue engineering. MSCs were seeded on the construct and exposed to adipogenic differentiation medium. Cell invasion was detected by two-photon microscopy, proliferation was measured by the PrestoBlue assay. Successful adipogenesis was demonstrated using Oil Red O staining as well as measurement of secreted adipokines. In conclusion, the given microenvironment featured optimal mechanical as well as biological properties for proliferation and differentiation of MSCs. Besides fat tissue, the textile-reinforced hydrogel system with adjustable mechanics could be a promising platform for future fabrication of versatile soft tissues, such as cartilage, tendon, or muscle.

الإتاحة: https://doi.org/10.3390/ma13163518Test
https://cris.maastrichtuniversity.nl/en/publications/a4f0e3c9-c683-4f19-9134-8eb307e7ab22Test

المؤلفون: Kneser, Ulrich, Bigdeli, Amir K, Himmler, Joerg P, Eyüpoglu, Ilker Y, Ganslandt, Oliver, Hirsch, Almut, Schmidt, Volker J, Beier, Justus P, Horch, Raymund E

المصدر: Kneser , U , Bigdeli , A K , Himmler , J P , Eyüpoglu , I Y , Ganslandt , O , Hirsch , A , Schmidt , V J , Beier , J P & Horch , R E 2015 , ' Comparison of the Ramirez technique for the closure of large open myelomeningocele defects with alternative methods ' , Journal of plastic, reconstructive & aesthetic surgery : JPRAS , vol. 68 , no. 12 , pp. 1675-82 . https://doi.org/10.1016/j.bjps.2015.08.002Test

مصطلحات موضوعية: Female, Humans, Infant, Newborn, Male, Meningomyelocele/surgery, Neurosurgical Procedures/methods, Plastic Surgery Procedures/methods, Retrospective Studies, Treatment Outcome

الوصف: BACKGROUND: To compare the Ramirez technique for the operative closure of large open myelomeningocele defects with conventional closure techniques in newborns. We hypothesized that the immediate surgical treatment with the Ramirez technique is superior to prior used operative techniques. METHODS: From 2003 to 2010, 23 children (8 female, 15 male) underwent closure of large open myelomeningocele defects using the Ramirez technique (group A), while from 1993 to 2002, 23 children (6 female, 17 male) underwent conventional closure techniques (group B). All children were included in the retrospective analysis with a mean follow-up period of 3.4 years. RESULTS: Perioperative variables were similar in both groups (P = ns). There were no hospital deaths in both groups. The operation time was significantly higher in group A (228.7 ± 76.8 versus 157.8 ± 70.3 min, P = 0.003). Mean length of hospital stay was significantly lower in group A (30.7 ± 16.4 days versus 52.0 ± 38.5; P = 0.02). Postoperative complication rate was significantly lower in group A (P = 0.01). Beyond postoperative day 10, liquor fluid leakage was significantly lower in group A (P = 0.05). During follow-up, there were no complications in group A. In group B, 2 children developed liquor fistulas. CONCLUSIONS: The Ramirez technique allows efficient and safe closure of large open myelomeningocele defects and reduces incidence of postoperative liquor fistulae. The increased operation time and surgical efforts seem to be justified. Treatment of large myelomeningocele defects requires an interdisciplinary team including paediatrician-neonatologists, neurosurgeons and plastic surgeons.

الإتاحة: https://doi.org/10.1016/j.bjps.2015.08.002Test
https://curis.ku.dk/portal/da/publications/comparison-of-the-ramirez-technique-for-the-closure-of-large-open-myelomeningocele-defects-with-alternative-methodsTest(b36cf18f-afa4-4e4a-90f4-ca5947012cde).html

المؤلفون: Kneser, Ulrich, Beier, Justus P, Schmitz, Marweh, Arkudas, Andreas, Dragu, Adrian, Schmidt, Volker J, Kremer, Thomas, Horch, Raymund E

المصدر: Kneser , U , Beier , J P , Schmitz , M , Arkudas , A , Dragu , A , Schmidt , V J , Kremer , T & Horch , R E 2014 , ' Zonal perfusion patterns in pedicled free-style perforator flaps ' , Journal of plastic, reconstructive & aesthetic surgery : JPRAS , vol. 67 , no. 1 , pp. e9-17 . https://doi.org/10.1016/j.bjps.2013.09.006Test

مصطلحات موضوعية: Adult, Aged, Female, Hemoglobins/metabolism, Humans, Laser-Doppler Flowmetry, Male, Middle Aged, Oxygen/blood, Perforator Flap/blood supply, Prospective Studies, Regional Blood Flow, Spectrophotometry, Young Adult

الوصف: INTRODUCTION: Local perforator flaps have become a standard procedure in reconstructive surgery. They allow the transfer of large tissue units with minimal donor-site morbidity. However, clinical studies on flap perfusion changes over time are lacking. The aim of this study was to investigate the perfusion of free-style single perforator flaps with an eccentrically located main perforator by combined laser Doppler spectrophotometry. PATIENTS AND METHODS: Ten patients (six male, four female, 29-71 years) were included in this prospective clinical study. All flaps were based on one perforator. Flaps were harvested from the trunk (n = 6) or the proximal upper or lower extremity (n = 4). Flap perfusion was assessed using a combined laser Doppler spectrophotometry (CLDS) device (O2C, Oxygen to See, LEA Medizintechnik, Giessen, Germany) at days 0, 1, 7 and 14 in different zones. RESULTS: Flap dimensions were 18.6 ± 4.7 × 7.2 ± 1.6 cm. Two flaps developed minor tip necroses (<10%), eight flaps survived completely. CLDS proved to be very sensitive for the detection of regional perfusion problems. A considerable perfusion gradient was observed at days 0 and 1. Here, reduced blood flow and post-capillary oxygen saturations were found at the tip when compared to the region above main perforator (RAMP). Blood flow remained stable proximally while it improved significantly from day 1 to 14 at the tip region. CONCLUSION: CLDS is an effective method for objective evaluation of flap perfusion. Although distal flap perfusion is diminished initially, the majority of perforator flaps with eccentrically located perforators survive completely. Obviously, flap perfusion improved between days 1 and 14. This clinical finding might be explained by reorganisation of the vascular system with opening of so-called connecting or choke vessels. This knowledge might influence decision making in perforator flap surgery.

الإتاحة: https://doi.org/10.1016/j.bjps.2013.09.006Test
https://curis.ku.dk/portal/da/publications/zonal-perfusion-patterns-in-pedicled-freestyle-perforator-flapsTest(67af0725-22fc-4052-881d-78c040c08875).html

المؤلفون: Schmidt, Volker J, Horch, Raymund E, Dragu, Adrian, Beier, Justus P, Eyüpoglu, Ilker Y, Hirsch, Almut, Kneser, Ulrich

المصدر: Schmidt , V J , Horch , R E , Dragu , A , Beier , J P , Eyüpoglu , I Y , Hirsch , A & Kneser , U 2012 , ' Myocutaneous propeller flap based on the superior gluteal artery (SGA) for closure of large lumbosacral meningomyelocoele defects : a case report ' , Journal of plastic, reconstructive & aesthetic surgery : JPRAS , vol. 65 , no. 4 , pp. 521-4 . https://doi.org/10.1016/j.bjps.2011.08.018Test

مصطلحات موضوعية: Arteries, Child, Preschool, Humans, Infant, Newborn, Lumbosacral Region, Male, Meningomyelocele/surgery, Plastic Surgery Procedures/methods, Surgical Flaps/blood supply

الوصف: Early and reliable closure of large meningomyelocoele defects in newborns is critical to prevent severe infectious complications and neurologic deterioration. Here, we introduce a new surgical method for the reconstruction of large horizontal meningomyelocoele defects, in which we use unilateral myocutaneous tissue based on the superior gluteal artery (SGA) as a propellar flap. This procedure permits a tension-free and durable multilayer closure of difficult, large-scale horizontal defects that cannot adequately be treated by conventional myocutaneous advancement flaps. The technique is reliable and straightforward and requires no skin grafts or relaxing incisions. The SGA-based myocutaneous propeller flap might be a promising alternative for complex meningomyelocoele reconstructions in the future.

الإتاحة: https://doi.org/10.1016/j.bjps.2011.08.018Test
https://curis.ku.dk/portal/da/publications/myocutaneous-propeller-flap-based-on-the-superior-gluteal-artery-sga-for-closure-of-large-lumbosacral-meningomyelocoele-defectsTest(7de0139d-2bae-4118-b358-3a444f5f9adf).html