دورية أكاديمية
5-HT(2B) receptor-mediated calcium release from ryanodine-sensitive intracellular stores in human pulmonary artery endothelial cells
| العنوان: | 5-HT(2B) receptor-mediated calcium release from ryanodine-sensitive intracellular stores in human pulmonary artery endothelial cells |
|---|---|
| المؤلفون: | Ullmer, C., Boddeke, H.G.W.M., Schmuck, K., Lubbert, H. |
| المصدر: | Ullmer , C , Boddeke , H G W M , Schmuck , K & Lubbert , H 1996 , ' 5-HT(2B) receptor-mediated calcium release from ryanodine-sensitive intracellular stores in human pulmonary artery endothelial cells ' , British Journal of Pharmacology , vol. 117 , no. 6 , pp. 1081-1088 . |
| سنة النشر: | 1996 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | 5-HT(2B) receptor, Heparin, Human pulmonary artery endothelial cells, Ryanodine receptor, 4 iodo 2,5 dimethoxyamphetamine, calcium ion, cyclic AMP, guanine nucleotide binding protein, histamine, messenger RNA, phosphatidylinositide, ruthenium red, ryanodine, serotonin, serotonin 1D receptor, serotonin 2 agonist, serotonin 2B receptor, animal cell, animal tissue, artery endothelium, article, calcium cell level, calcium transport, controlled study, human, phosphoinositide metabolism, priority journal, pulmonary artery, receptor gene, reverse transcription polymerase chain reaction |
| الوصف: | 1. We have characterized the 5-hydroxytryptamine (5-HT)-induced calcium signalling in endothelial cells from the human pulmonary artery. Using RT-PCR we show, that of all cloned G-protein coupled 5-HT receptors, these cells express only 5-HT(1Dβ), 5-HT(2B) and little 5-HT4receptor mRNA. 2. In endothelial cells 5-HT inhibits the formation of adenosine 3':5'-cyclic monophosphate (cyclic AMP) via 5-HT(1Dβ) receptors but fails to activate phosphoinositide (PI) turnover. However, the latter pathway is strongly activated by histamine. 3. Despite the lack of detectable inositol phosphate (IP) formation in human pulmonary artery endothelial cells, 5-HT (pD2= 5.82 ± 0.06, n = 6) or the selective 5-HT2agonist, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) (pD2= 5.66 ± 0.03, n = 7) elicited transient calcium signals comparable to those evoked by histamine (pD2= 6.44 ± 0.01, n = 7). Since 5-HT(2A) and 5-HT(2C) receptor mRNAs are not detectable in pulmonary artery endothelial cells, activation of 5-HT(2B) receptors is responsible for the transient calcium release. The calcium transients are independent of the inhibition of adenylate cyclase, since DOI does not stimulate 5-HT(1Dβ) receptors. 4. Both, the 5-HT- and histamine-stimulated calcium signals were also observed when the cells were placed in calcium-free medium. This indicates that 5-HT triggers calcium release from intracellular stores. 5. Heparin is an inhibitor of the IP3-activated calcium release channels on the endoplasmic reticulum. Intracellular infusion of heparin through patch pipettes in voltage clamp experiments failed to block 5-HT-induced calcium signals, whereas it abolished the histamine response. This supports the conclusion that the 5-HT-induced calcium release is independent of IP3formation. 6. Unlike the histamine response, the 5-HT response was sensitive to micromolar concentrations of ryanodine and, to a lesser extent, ruthenium red. This implies that 5-HT(2B) receptors trigger calcium release from a ryanodine-sensitive calcium pool. 7. It ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://research.rug.nl/en/publications/f2218444-ac17-49a2-9d89-efcbe9d84627Test |
| الإتاحة: | https://hdl.handle.net/11370/f2218444-ac17-49a2-9d89-efcbe9d84627Test https://research.rug.nl/en/publications/f2218444-ac17-49a2-9d89-efcbe9d84627Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.EE9909FF |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |