دورية أكاديمية
Formation of aggresome-like structures in herpes simplex virus type 2-infected cells and a potential role in virus assembly
| العنوان: | Formation of aggresome-like structures in herpes simplex virus type 2-infected cells and a potential role in virus assembly |
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| المؤلفون: | Nozawa, Naoki, Yamauchi, Yohei, Ohtsuka, Kenzo, Kawaguchi, Yasushi, Nishiyama, Yukihiro |
| المصدر: | Nozawa , N , Yamauchi , Y , Ohtsuka , K , Kawaguchi , Y & Nishiyama , Y 2004 , ' Formation of aggresome-like structures in herpes simplex virus type 2-infected cells and a potential role in virus assembly ' , Experimental Cell Research , vol. 299 , no. 2 , pp. 486-97 . https://doi.org/10.1016/j.yexcr.2004.06.010Test |
| سنة النشر: | 2004 |
| المجموعة: | University of Bristol: Bristol Reserach |
| مصطلحات موضوعية: | Animals, Antineoplastic Agents, Biological Transport, Cercopithecus aethiops, Gene Expression Regulation, Herpesvirus 2, Human, Microtubule-Organizing Center, Microtubules, Nocodazole, Organelles, Vero Cells, Vimentin, Viral Structural Proteins, Virus Assembly, Virus Replication, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't |
| الوصف: | Herpes simplex virus (HSV) is a large, enveloped DNA virus that replicates in the nucleus and is assembled in the cytoplasm to the mature infectious virion. In this study, we present evidence that, in HSV-2-infected cells, some tegument proteins (UL46 and VP16) and newly synthesized nucleocapsids accumulate in a juxtanuclear domain sharing characteristics with aggresomes, cellular structures formed in response to misfolded proteins [J. Cell Biol. 146 (1999) 1239, J. Cell Biol. 143 (1998) 2010]. The juxtanuclear domains (aggresome-like structures) induced by HSV-2 infection localize to the microtubule organizing center (MTOC) where the clustering mitochondria, Golgi-derived vesicles, and cellular chaperones including heat shock protein (Hsp)40 and Hsp70 were recruited. Formation of aggresome-like structures was blocked by the presence of microtubule-disassembling drug nocodazole, indicating that microtubule-dependent transport may be involved in the accumulation of viral and cellular proteins at these sites in HSV-2-infected cells. These features are similar to those governing the formation of aggresomes. In contrast to aggresomes, however, the vimentin cage surrounding the MTOC was not observed with the aggresome-like structures in HSV-2-infected cells, and the maintenance of these structures required an intact microtubular network. Disruption of the aggresome-like structures by nocodazole treatment led to a low but consistent effect (10-fold decrease) on the production of intracellular infectious particles. These results suggest that aggresome-like structures do not play a critical but augmentary role in HSV-2 replication. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.yexcr.2004.06.010 |
| الإتاحة: | https://doi.org/10.1016/j.yexcr.2004.06.010Test https://hdl.handle.net/1983/489020d3-9ae7-46f4-b73c-591e875c6618Test https://research-information.bris.ac.uk/en/publications/489020d3-9ae7-46f4-b73c-591e875c6618Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.651EDE06 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.yexcr.2004.06.010 |
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