دورية أكاديمية
Neutrophil-mediated type IV collagen degradation is elevated in patients with mild endoscopic ulcerative colitis reflecting early mucosal destruction
| العنوان: | Neutrophil-mediated type IV collagen degradation is elevated in patients with mild endoscopic ulcerative colitis reflecting early mucosal destruction |
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| المؤلفون: | Alexdottir, Marta S., Pehrsson, Martin, Domislovic, Viktor, Godskesen, Line E., Krag, Aleksander, Kjeldsen, Jens, Brinar, Marko, Barisic, Ana, Bay-Jensen, Anne Christine, Karsdal, Morten A., Krznaric, Zeljko, Mortensen, Joachim H. |
| المصدر: | Alexdottir , M S , Pehrsson , M , Domislovic , V , Godskesen , L E , Krag , A , Kjeldsen , J , Brinar , M , Barisic , A , Bay-Jensen , A C , Karsdal , M A , Krznaric , Z & Mortensen , J H 2024 , ' Neutrophil-mediated type IV collagen degradation is elevated in patients with mild endoscopic ulcerative colitis reflecting early mucosal destruction ' , Scientific Reports , vol. 14 , 1641 . https://doi.org/10.1038/s41598-024-52208-yTest |
| سنة النشر: | 2024 |
| المجموعة: | University of Southern Denmark: Research Output / Syddansk Universitet |
| مصطلحات موضوعية: | Basement Membrane/metabolism, Biomarkers/metabolism, Colitis, Ulcerative/metabolism, Collagen Type IV/metabolism, Humans, Neutrophils/metabolism |
| الوصف: | Neutrophils play a significant role in sustaining chronic inflammation in Inflammatory Bowel Disease. The intestinal basement membrane acts as a barrier for immunological homeostasis, where the α3 and α4 chains of type IV collagen are expressed on the mucosal surface. We wanted to develop a biomarker reflecting early tissue injury, providing an opportunity for intervention. Two competitive enzyme-linked immunosorbent assays (ELISAs) quantifying human neutrophil elastase (HNE) degraded neo-epitopes of COL4A3 and COL4A4 were developed and investigated in two observational cohorts (n = 161, n = 100). A biomarker of MMP-mediated degradation of COL4A1 (C4M) was used for comparison. In Cohort 1, patients with mild endoscopic ulcerative colitis showed elevated levels of C4A3-HNE compared to those with severe disease. C4M had a strong positive correlation with disease activity. C4A3-HNE/C4M provided superior discrimination between mild and severe endoscopic disease and negatively correlated to disease activity. In Cohort 2, C4A4-HNE and C4A4-HNE/C4M showed similar trends. C4A3-HNE and C4A4-HNE possibly reflect early intestinal tissue injury. Combining the markers with a biomarker of another α-chain of the same collagen provides information on two distinct stages of mucosal damage. These biomarkers may be used to monitor disease flare-up in patients in remission, reducing the need for frequent endoscopic procedures. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://portal.findresearcher.sdu.dk/da/publications/7a39e977-0867-492a-86ad-76817b93a8a3Test |
| DOI: | 10.1038/s41598-024-52208-y |
| الإتاحة: | https://doi.org/10.1038/s41598-024-52208-yTest https://portal.findresearcher.sdu.dk/da/publications/7a39e977-0867-492a-86ad-76817b93a8a3Test https://findresearcher.sdu.dk/ws/files/254006912/s41598-024-52208-y.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F6DF95DF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-024-52208-y |
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