المؤلفون: Pistollato, Francesca, Bal-Price, Anna, Coecke, Sandra, Parvatam, Surat, Pamies, David, Czysz, Katherine, Hao, Jie, Kee, Kehkooi, Teo, Adrian Kee Keong, Niu, Shuaishuai, Wilmes, Anja, Smirnova, Lena, Freund, Christian, Mummery, Christine, Stacey, Glyn

المصدر: Pistollato , F , Bal-Price , A , Coecke , S , Parvatam , S , Pamies , D , Czysz , K , Hao , J , Kee , K , Teo , A K K , Niu , S , Wilmes , A , Smirnova , L , Freund , C , Mummery , C & Stacey , G 2022 , ' Quality criteria for in vitro human pluripotent stem cell-derived models of tissue-based cells ' , Reproductive Toxicology , vol. 112 , pp. 36-50 . https://doi.org/10.1016/j.reprotox.2022.06.003Test

مصطلحات موضوعية: 3D, Differentiation, Ectoderm, Endoderm, GCCP, Human pluripotent stem cells, In vitro toxicology, iPSCs, Mesoderm, Quality control criteria, Readiness level, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being

وصف الملف: application/pdf

العلاقة: https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test

الإتاحة: https://doi.org/10.1016/j.reprotox.2022.06.003Test
https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://hdl.handle.net/1871.1/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://research.vu.nl/ws/files/170377697/Quality_criteria_for_in_vitro_human_pluripotent_stem_cell_derived_models_of_tissue_based_cells.pdfTest
http://www.scopus.com/inward/record.url?scp=85133253702&partnerID=8YFLogxKTest
http://www.scopus.com/inward/citedby.url?scp=85133253702&partnerID=8YFLogxKTest

المؤلفون: Pistollato, Francesca, Bal-Price, Anna, Coecke, Sandra, Parvatam, Surat, Pamies, David, Czysz, Katherine, Hao, Jie, Kee, Kehkooi, Teo, Adrian Kee Keong, Niu, Shuaishuai, Wilmes, Anja, Smirnova, Lena, Freund, Christian, Mummery, Christine, Stacey, Glyn

المصدر: Pistollato , F , Bal-Price , A , Coecke , S , Parvatam , S , Pamies , D , Czysz , K , Hao , J , Kee , K , Teo , A K K , Niu , S , Wilmes , A , Smirnova , L , Freund , C , Mummery , C & Stacey , G 2022 , ' Quality criteria for in vitro human pluripotent stem cell-derived models of tissue-based cells ' , Reproductive Toxicology , vol. 112 , pp. 36-50 . https://doi.org/10.1016/j.reprotox.2022.06.003Test

مصطلحات موضوعية: 3D, Differentiation, Ectoderm, Endoderm, GCCP, Human pluripotent stem cells, In vitro toxicology, iPSCs, Mesoderm, Quality control criteria, Readiness level, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being

وصف الملف: application/pdf

العلاقة: https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test

الإتاحة: https://doi.org/10.1016/j.reprotox.2022.06.003Test
https://research.vu.nl/en/publications/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://hdl.handle.net/1871.1/4644d3eb-6902-479b-90f3-4e349a4554e2Test
https://research.vu.nl/ws/files/170377697/Quality_criteria_for_in_vitro_human_pluripotent_stem_cell_derived_models_of_tissue_based_cells.pdfTest
http://www.scopus.com/inward/record.url?scp=85133253702&partnerID=8YFLogxKTest
http://www.scopus.com/inward/citedby.url?scp=85133253702&partnerID=8YFLogxKTest

المؤلفون: Perera, Marta, Nissen, Silas Boye, Proks, Martin, Pozzi, Sara, Monteiro, Rita S., Trusina, Ala, Brickman, Joshua M.

المصدر: Perera , M , Nissen , S B , Proks , M , Pozzi , S , Monteiro , R S , Trusina , A & Brickman , J M 2022 , ' Transcriptional heterogeneity and cell cycle regulation as central determinants of Primitive Endoderm priming ' , eLife , vol. 11 , e78967 . https://doi.org/10.7554/eLife.78967Test

مصطلحات موضوعية: embryonic stem cells, endoderm, differentiation, selection, cell cycle, heterogeneity, Mouse, EMBRYONIC STEM-CELLS, G1 PHASE, GROUND-STATE, SELF-RENEWAL, PLURIPOTENCY, SEGREGATION, EXPRESSION, EPIBLAST, CULTURE

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.7554/eLife.78967Test
https://curis.ku.dk/portal/da/publications/transcriptional-heterogeneity-and-cell-cycle-regulation-as-central-determinants-of-primitiveTest-endoderm-priming(73d7ca8d-f9f8-4fba-a244-54473c30e38b).html
https://curis.ku.dk/ws/files/318799585/elife_78967_v3.pdfTest

المؤلفون: Miller, Alyssa J., Dye, Briana R., Ferrer-Torres, Daysha, Hill, David R., Overeem, Arend W., Shea, Lonnie D., Spence, Jason R.

المصدر: Miller , A J , Dye , B R , Ferrer-Torres , D , Hill , D R , Overeem , A W , Shea , L D & Spence , J R 2019 , ' Generation of lung organoids from human pluripotent stem cells in vitro ' , Nature protocols , vol. 14 , no. 2 , pp. 518-540 . https://doi.org/10.1038/s41596-018-0104-8Test

مصطلحات موضوعية: PROGENITOR CELLS, EFFICIENT DERIVATION, AIRWAY EPITHELIUM, ANIMAL-MODELS, DISEASE, DIFFERENTIATION, ENDODERM

وصف الملف: application/pdf

العلاقة: https://research.rug.nl/en/publications/6acd069c-e99e-4df0-98a8-7ec6e68cfc31Test

الإتاحة: https://doi.org/10.1038/s41596-018-0104-8Test
https://hdl.handle.net/11370/6acd069c-e99e-4df0-98a8-7ec6e68cfc31Test
https://research.rug.nl/en/publications/6acd069c-e99e-4df0-98a8-7ec6e68cfc31Test
https://pure.rug.nl/ws/files/91994105/s41596_018_0104_8.pdfTest

المؤلفون: Sambathkumar, Rangarajan, Kalo, Eric, Van Rossom, Rob, Faas, Marijke M., de Vos, Paul, Verfaillie, Catherine M.

المصدر: Sambathkumar , R , Kalo , E , Van Rossom , R , Faas , M M , de Vos , P & Verfaillie , C M 2016 , ' Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts ' , Stem cells international , vol. 2016 , 7654321 . https://doi.org/10.1155/2016/7654321Test

مصطلحات موضوعية: Reprogramming can occur by the introduction of key transcription factors (TFs) as well as by epigenetic changes. We demonstrated, that histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) combined with a chromatin remodeling medium (CRM), induced expression of a number of definitive endoderm and early and late pancreatic marker genes. When CRM was omitted, endoderm/pancreatic marker genes were not induced. Furthermore, treatment with DNA methyltransferase inhibitor (DNMTi), 5-azacytidine (5AZA) CRM did not affect gene expression changes, and when 5AZA was combined with TSA, no further increase, in gene expression of endoderm, pancreatic endoderm, and endocrine markers was seen over levels induced with TSA alone, Interestingly, TSA-CRM did not affect expression of pluripotency and hepatocyte genes but induced some mesoderm transcripts, Upon removal of TSA-CRM, the endoderm/pancreatic gene expression profile returned to baseline. Our findings underscore the, role epigenetic modification in transdifferentiation of one somatic cell into another. However, full reprogramming of fibroblasts to beta-cells will require combination of this approach with TF overexpression and/or culture of the partially reprogrammed cells under beta-cell specific conditions, INSULIN-SECRETING CELLS, PLURIPOTENT STEM-CELLS, BETA-CELLS, IN-VITRO, SMALL MOLECULES, DIFFERENTIATION, TRICHOSTATIN, CONVERSION, CHROMATIN, VIVO

وصف الملف: application/pdf

العلاقة: https://research.rug.nl/en/publications/bf1df5bc-02cc-4e6f-9ce3-58335ac1f159Test

الإتاحة: https://doi.org/10.1155/2016/7654321Test
https://hdl.handle.net/11370/bf1df5bc-02cc-4e6f-9ce3-58335ac1f159Test
https://research.rug.nl/en/publications/bf1df5bc-02cc-4e6f-9ce3-58335ac1f159Test
https://pure.rug.nl/ws/files/35733716/7654321.pdfTest