دورية أكاديمية
Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks
| العنوان: | Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks |
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| المؤلفون: | Gutiérrez, Ana, Rencoret, Jorge, Cadena, Edith M., Rico, Alejandro, Barth, Dorothee, del Rio, José C., Martínez, Ángel T. |
| المصدر: | Gutiérrez , A , Rencoret , J , Cadena , E M , Rico , A , Barth , D , del Rio , J C & Martínez , Á T 2012 , ' Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks ' , Bioresource Technology , vol. 119 , pp. 114-122 . https://doi.org/10.1016/j.biortech.2012.05.112Test |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Enzymatic deconstruction, laccase, bioethanol, lignocellulose, 2D NMR, /dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energy, name=SDG 7 - Affordable and Clean Energy |
| الوصف: | The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood ( Eucalyptus globulus ) and non-wood ( Pennisetum purpureum ) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum ) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://cris.vtt.fi/en/publications/adcbc450-5cf4-40fd-b8b8-bc6bb2684a0aTest |
| DOI: | 10.1016/j.biortech.2012.05.112 |
| الإتاحة: | https://doi.org/10.1016/j.biortech.2012.05.112Test https://cris.vtt.fi/en/publications/adcbc450-5cf4-40fd-b8b8-bc6bb2684a0aTest |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.56C3BA31 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.biortech.2012.05.112 |
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