Multimodal Nonlinear Microscope based on a Compact Fiber-format Laser Source

التفاصيل البيبلوغرافية
العنوان: Multimodal Nonlinear Microscope based on a Compact Fiber-format Laser Source
المؤلفون: Crisafi, Francesco, Kumar, Vikas, Perri, Antonio, Marangoni, Marco, Cerullo, Giulio, Polli, Dario
المصدر: Spectrochimica Acta Part A vol. 188, pages 135-140 (2018)
سنة النشر: 2017
المجموعة: Physics (Other)
مصطلحات موضوعية: Physics - Optics
الوصف: We present a multimodal non-linear optical (NLO) laser-scanning microscope, based on a compact fiber-format excitation laser and integrating coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and two-photon-excitation fluorescence (TPEF) on a single platform. We demonstrate its capabilities in simultaneously acquiring CARS and SRS images of a blend of 6-{\mu}m poly(methyl methacrylate) beads and 3-{\mu}m polystyrene beads. We then apply it to visualize cell walls and chloroplast of an unprocessed fresh leaf of Elodea aquatic plant via SRS and TPEF modalities, respectively. The presented NLO microscope, developed in house using off-the-shelf components, offers full accessibility to the optical path and ensures its easy re-configurability and flexibility.
نوع الوثيقة: Working Paper
DOI: 10.1016/j.saa.2017.06.055
الوصول الحر: http://arxiv.org/abs/1707.04545Test
رقم الانضمام: edsarx.1707.04545
قاعدة البيانات: arXiv