Patent
Establishment of rhesus monkey model of autoimmunity type 1 diabetes
| العنوان: | Establishment of rhesus monkey model of autoimmunity type 1 diabetes |
|---|---|
| Patent Number: | 9,186,421 |
| تاريخ النشر: | November 17, 2015 |
| Appl. No: | 13/637125 |
| Application Filed: | March 26, 2010 |
| مستخلص: | Use of low dose streptozocin in the preparation of an animal model for screening drugs for treatment of antoimmune type 1 diabetes is disclosed, in which streptozocin is administrated intravenously at a dose of 15-30 mg/kg per time for 5 days and administrated again on the 7th day and 14th day after last time of offering drug. A method for preparing rhesus monkey model of autoimmune type 1 diabetes and autoimmune type 1 diabetes animal model obtained are also disclosed. |
| Inventors: | Lu, Yanrong (Chengdu, CN); Cheng, Jingqiu (Chengdu, CN); Deng, Shaoping (Chengdu, CN); Chen, Younan (Chengdu, CN); Tian, Bole (Chengdu, CN); Mai, Gang (Chengdu, CN); Ren, Yan (Chengdu, CN); Wang, Li (Chengdu, CN); Li, Hongxia (Chengdu, CN); Wei, Lingling (Chengdu, CN); Jin, Xi (Chengdu, CN); Qiao, Chaofeng (Chengdu, CN); Zhang, Wensheng (Chengdu, CN); He, Sirong (Chengdu, CN); Zeng, Li (Chengdu, CN) |
| Assignees: | West China Hospital, Sichuan University (Sichuan, CN) |
| Claim: | 1. A method of preparing a rhesus monkey model for screening drugs for treatment of autoimmune type 1 diabetes, the method comprising: intravenously administering streptozotocin to a rhesus monkey at a dose of 20-30 mg/kg per injection per diem for 5 consecutive days, the first injection being given on day 1; and intravenously administering streptozotocin to the rhesus monkey at a dose of 20-30 mg/kg per injection per diem on the 12 th day and the 19 th day after day 1 of the 5-day administration, wherein the rhesus monkey exhibits a fasting glucose level greater than 11.1 mmol/L, a C-peptide level less than 0.3 nM, and has anti-islet cell antibodies and anti-insulin antibodies. |
| Claim: | 2. An autoimmune type 1 diabetes rhesus monkey model prepared by the method of claim 1 , wherein the rhesus monkey exhibits a fasting glucose level greater than 11.1 mmol/L, a C-peptide level less than 0.3 nM, and has anti-islet cell antibodies and anti-insulin antibodies. |
| Claim: | 3. A method of screening drugs for treatment of autoimmune type 1 diabetes, comprising the steps of: a) administering a candidate drug into the rhesus monkey model of claim 2 ; b) evaluating the candidate drug for treatment of autoimmune type 1 diabetes. |
| Patent References Cited: | WO 2008010690 January 2008 |
| Other References: | Qiao et al. Induction of Diabetes in Rhesus Monkeys and Establishment of Insulin Administration Strategy. Transplantation Proceedings, 2009. 41:413-417. cited by examiner Koulmanda et al. The Effect of Low Versus High Dose of Streptozotocin in Cynomolgus Monkeys (Macaca fascilularis). American Journal of Transplantation, 2003. 3:267-272. cited by examiner Lu et al, The Evidences of Induced Autoimmue Diabetes in Primates: Establishment of a Preclinical Large Animal Model of T1DM. Abstract. Xenotransplantation, (Sep.-Oct. 2009) vol. 16, No. 5, pp. 348. Abstract No. JOINT-O-1.5. Meeting Info: 2009 Joint Meeting of the International Pancreas and Islet Transplant Association (IPITA) and the I. cited by examiner Elias et al. Autoimmune Diabetes Induced by the Beta-Cell Toxin STZ. Diabetes, 1994. 43: 992-998. cited by examiner El-Housseiny et al. Studies on the Induction of Stable and Prolonged Hyperglycemia by SubDiabetogenic Dose of Streptozotocin (STZ). Journal of Drug Research, Egypt, 1987. 17(1-2): 45-51. cited by examiner Jones et al. Streptozotocin Diabetes in the Monkey: Plasma levels of Glucose, Insulin, Glucagon, and Somatostatin, with Corresponding Morphometruc Analysis of Islet Endocrine cells. Diabetes, 1980. 29(7)536-46. Abstract only. cited by examiner Wright et al, Synergistic Effect of Adjuvants, Endotoxin, and Fasting on Induction of Diabetes With Multiple Low Doses of Streptozocin in Rats. Diabetes, 1998. 37(1):112-118. cited by examiner Gaur, Lakshmi K. Nonhuman Primate Models for Islet Transplantation in Type 1 Diabetes Research. ILAR Journal, 2004. 45(3):324-333. cited by examiner |
| Assistant Examiner: | Aron, Kimberly A |
| Primary Examiner: | Leavitt, Maria |
| Attorney, Agent or Firm: | Cesari and McKenna, LLP |
| رقم الانضمام: | edspgr.09186421 |
| قاعدة البيانات: | USPTO Patent Grants |
| الوصف غير متاح. |