التفاصيل البيبلوغرافية
| العنوان: |
P.122 Unbiased whole brain circuit interrogation reveals neurons restoring walking after spinal cord injury |
| المؤلفون: |
Cho, N, Squair, J, James, N, Baud, L, Leonhartsberger, A, Sveistyte, K, Galan, K, Barraud, Q, Goubran, M, Batti, L, Pages, S, Gautier, M, Hutson, T, Kathe, C, Bichat, A, Rizzo, O, Hodara, M, Bloch, J, Courtine, G |
| المصدر: |
The Canadian Journal of Neurological Sciences; June 2022, Vol. 49 Issue: Supplement 1 pS40-S41, 2p |
| مستخلص: |
Background: There is presently no cure for locomotor deficits after spinal cord injury (SCI). Very few therapies effectively target the brain due to poor understanding of the brain’s role post-SCI. Newly developed tissue clearing techniques have permitted unbiased three-dimensional circuit analysis, opening new opportunities for SCI-related brain interrogation. Methods: We established a novel brain interrogation pipeline by optimizing mouse brain clearing, imaging, and atlas registration. We leveraged a spontaneous recovery lateral hemisection model to analyze whole brain cell activity and connectivity with the lumbar cord using cFos immunolabelling and virus-mediated projection tracing. We identified a functionally and anatomically dynamic region correlating with recovery and interrogated its locomotor role with optogenetics. We assessed deep brain electrical stimulation (DBS) of this region in a more clinically relevant rat contusion SCI using an established bipedal robotic interface. Results: We unexpectedly uncovered the lateral hypothalamus (LH) to functionally and anatomically correlate with recovery. LHVglut2optogenetic stimulation significantly augmented locomotor function. LH DBS in rats acutely robustly augmented bipedal locomotion post-SCI. Conclusions: This is the first demonstration of the LH’s role in locomotion post-SCI and is a novel DBS target that robustly augmented locomotor function, dependent on LH glutamatergic cells. LH DBS may be a promising intervention in humans. |
| قاعدة البيانات: |
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