التفاصيل البيبلوغرافية
| العنوان: |
The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2. |
| المؤلفون: |
Shoichi WAKITANI, Daigo YOKOI, Yuichi HIDAKA, Koichiro NISHINO |
| المصدر: |
Journal of Veterinary Medical Science; Feb2017, Vol. 79 Issue 2, p230-237, 8p |
| مصطلحات موضوعية: |
BONE growth, DNA methylation, GENE expression, TRANSCRIPTION factors, NUCLEOTIDE sequence, GENETICS |
| مستخلص: |
Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2.7 to -2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2-DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Veterinary Medical Science is the property of Japanese Society of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
Full Text Finder